首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for Confirmation of AMPLICOR PCR-Positive Samples and Comparison of Its Diagnostic Performance According to Storage Conditions and Preparation of Endocervical Specimens
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Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for Confirmation of AMPLICOR PCR-Positive Samples and Comparison of Its Diagnostic Performance According to Storage Conditions and Preparation of Endocervical Specimens

机译:罗氏淋球菌淋病奈瑟氏菌16S rRNA PCR的评估,以确认AMPLICOR PCR阳性样品,并根据保存条件和宫颈内膜标本的诊断性能比较

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摘要

The AMPLICOR PCR was used to detect Neisseria gonorrhoeae in endocervical specimens. A 16S rRNA PCR performed on N. gonorrhoeae-positive samples showed sensitivities of 73.2, 64.3, and 94.4% for samples treated directly with AMPLICOR lysis buffer, samples suspended in 2-sucrose phosphate, and samples suspended in diluted phosphate-buffered saline, respectively.
机译:AMPLICOR PCR用于检测宫颈内标本中的淋病奈瑟氏菌。在 N上进行16S rRNA PCR。淋球菌阳性样品对直接用AMPLICOR裂解缓冲液处理的样品,悬浮在2-蔗糖磷酸中的样品和悬浮在稀释的磷酸盐缓冲盐水中的样品的敏感性分别为73.2%,64.3和94.4%。

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