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首页> 外文期刊>Journal of Clinical Microbiology >Epidemiologic Genotyping of Methicillin-Resistant Staphylococcus aureus by Pulsed-Field Gel Electrophoresis at a University Hospital and Comparison with Antibiotyping and Protein A and Coagulase Gene Polymorphisms
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Epidemiologic Genotyping of Methicillin-Resistant Staphylococcus aureus by Pulsed-Field Gel Electrophoresis at a University Hospital and Comparison with Antibiotyping and Protein A and Coagulase Gene Polymorphisms

机译：在大学医院中通过脉冲场凝胶电泳对耐甲氧西林金黄色葡萄球菌进行流行病学基因分型，并与抗生物型分型和蛋白A和凝固酶基因多态性进行比较

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A total of 124 methicillin-resistant Staphylococcus aureus (MRSA) isolates were ascertained at the University Hospital of the Canary Islands between January 1997 and April 2000. Genotyping included pulsed-field gel electrophoresis (PFGE) (SmaI digestion) and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis for the coagulase (coa) and protein A (spa) genes. Antibiotic resistance was the main phenotypic marker correlated with genotyping results. Three main PFGE types were detected: A (with 12 subtypes), B (with 2 subtypes), and C. PFGE type A1 was the most commonly found (61% of isolates) and the one responsible for all the epidemic outbreaks. Other genetics markers used (coa and spa RFLPs) were significantly correlated with the PFGE types detected (P < 0.001). These PCR-RFLP assays were useful as molecular markers for a quick, preliminary study of MRSA outbreaks.
机译：在1997年1月至2000年4月之间，加那利群岛大学医院共确定了124株耐甲氧西林金黄色葡萄球菌（MRSA）分离株。基因分型包括脉冲场凝胶电泳（PFGE）（ Sma I消化）和PCR酶切片段长度多态性（PCR-RFLP）分析凝固酶（ coa ）和蛋白A（ spa ）基因。抗生素耐药性是与基因分型结果相关的主要表型标记。检测到三种主要的PFGE类型：A（具有12个亚型），B（具有2个亚型）和C。最常见的是PFGE A1型（占分离株的61％），并且是所有流行病爆发的原因。其他使用的遗传标记（ coa 和 spa RFLP）与检测到的PFGE类型显着相关（ P <0.001）。这些PCR-RFLP分析可用作MRSA爆发的快速，初步研究的分子标记。

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