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首页> 外文期刊>Journal of Clinical Microbiology >Macrolide Efflux Genes mef(A) and mef(E) Are Carried by Different Genetic Elements in Streptococcus pneumoniae
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Macrolide Efflux Genes mef(A) and mef(E) Are Carried by Different Genetic Elements in Streptococcus pneumoniae

机译：大环内酯外排基因mef（A）和mef（E）携带不同的遗传因子在肺炎链球菌中

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Susceptibilities to macrolides were evaluated in 267 Streptococcus pneumoniae isolates, of which 182 were from patients with invasive diseases and 85 were from healthy carriers. Of the 98 resistant isolates, 20 strains showed an M phenotype and carried mef. Strains that carried both mef(A) and mef(E) were found: 17 strains carried mef(A) and 3 carried mef(E). The characteristics of the strains carrying the mef genes and the properties of the mef-containing elements were studied. Strains carrying mef(A) belonged to serotype 14, were susceptible to all the antibiotics tested except erythromycin, and appeared to be clonally related by pulsed-field gel electrophoresis (PFGE). The three mef(E) strains belonged to different serotypes, showed different susceptibility profiles, and did not appear to be related by PFGE. The sequences of a fragment of the mef-containing element, which encompassed mef and the msr(A) homolog, were identical among the three mef(E)-positive strains and among the three mef(A)-positive strains, although there were differences between the sequences for the two variants at 168 positions. In all mef(A)-positive strains, the mef element was inserted in celB, which led to impairment of the competence of the strains. In line with insertion of the mef(E) element at a different site, the competence of the mef(E)-positive strains was maintained. Transfer of erythromycin resistance by conjugation was obtained from two of three mef(A) strains but from none of three mef(E) strains. Due to the important different characteristics of the strains carrying mef(A) or mef(E), we suggest that the distinction between the two genes be maintained.
机译：评价了267种肺炎链球菌对大环内酯类药物的敏感性，其中182种来自浸润性疾病患者，而85种来自健康携带者。在98株耐药菌株中，有20株表现出M表型并带有 mef 。发现同时带有 mef （A）和 mef （E）的菌株：17个带有 mef （A）的菌株和3个带有 mef （A）的菌株。 mef （E）。研究了携带 mef 基因的菌株的特征和包含 mef-元素的特性。携带 mef （A）的菌株属于血清型14，除红霉素外，对所有测试的抗生素均敏感，并且似乎与脉冲场凝胶电泳（PFGE）有克隆相关性。这三个 mef （E）菌株属于不同的血清型，表现出不同的敏感性分布，似乎与PFGE无关。包含 mef 和 msr （A）同源物的 mef-元素片段的序列在三个< em> mef （E）阳性菌株和三个 mef （A）阳性菌株中，尽管两个变体在168位的序列之间存在差异。在所有 mef （A）阳性菌株中，在 celB 中插入了 mef 元件，这导致了菌株的功能受损。与 mef （E）元件在不同位点的插入一致，维持了 mef （E）阳性菌株的能力。红霉素抗性的结合转移是从三个 mef （A）菌株中的两个获得的，但没有从三个 mef （E）菌株中获得的。由于携带 mef （A）或 mef （E）的菌株的重要不同特征，我们建议保持这两个基因之间的区别。

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