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首页> 外文期刊>Journal of Clinical Microbiology >Characterization of Shiga Toxin-Producing Escherichia coli O157:H7 Isolated in Italy and in France
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Characterization of Shiga Toxin-Producing Escherichia coli O157:H7 Isolated in Italy and in France

机译：在意大利和法国分离到的产志贺毒素的大肠杆菌O157：H7的表征

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Twenty-one Escherichia coli O157:H7 strains isolated in northern Italy from sporadic cases of hemolytic-uremic syndrome and from cattle and food were characterized by virulence gene analysis, pulsed-field gel electrophoresis (PFGE) of XbaI-digested DNA, enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR (ERIC-PCR), and antibiotic resistance patterns and compared to 18 strains isolated in France from human cases of diarrhea, cattle, and the environment. Strains isolated in Sicily (southern Italy) from a local farm (one strain) and from calves just imported from France (11 strains) and Spain (six strains) were also typed. Whereas the eae and hlyA genes were always detected, Shiga toxin gene (stx) analysis showed some differences related to geographic areas. Isolates from northern Italy showed a high frequency of stx1 and stx2, while strains isolated in France and from French and Spanish calves imported to Sicily more frequently possessed the stx2c gene. The majority of the strains isolated in northern Italy were also resistant to one or more antibiotics, while most of the strains isolated in France and Sicily were fully susceptible. ERIC-PCR analysis was not able to differentiate the strains. PFGE typing after XbaI DNA digestion produced a total of 54 distinct restriction endonuclease digestion profiles (REDPs) among the 57 strains. Phylogenetic analysis was unable to cluster REDPs according to geographic origin. All epidemiologically related isolates showed either identical or ≥91% similar REDPs. Our findings suggest a peculiar circulation of antibiotic-resistant, genetically unrelated strains in northern Italy.
机译：通过毒力基因分析，脉冲场凝胶电泳（PFGE）对意大利北部从偶发溶血性尿毒症综合征病例以及从牛和食物中分离出的21株大肠埃希菌O157：H7菌株进行了毒力基因分析，脉冲场凝胶电泳（PFGE） em> Xba I消化的DNA，基于肠细菌重复基因间共有序列（ERIC）序列的PCR（ERIC-PCR）和抗生素耐药性模式，并与法国从人腹泻，牛和环境。还对从当地农场（一种菌株）和刚从法国（11种菌株）和西班牙（六种菌株）进口的小牛分离出的菌株进行了分型。尽管总是检测到 eae 和 hlyA 基因，但志贺毒素基因（ stx ）分析显示出与地理区域相关的一些差异。来自意大利北部的分离株显示出 stx 1 和 stx 2 的高频率，而菌株在法国和进口到西西里岛的法国和西班牙牛犊更常具有 stx 2c 基因。在意大利北部分离出的大多数菌株也对一种或多种抗生素具有抗性，而在法国和西西里分离出的大多数菌株则完全易感。 ERIC-PCR分析无法区分菌株。 Xem DNA消化后的PFGE分型在57个菌株中产生了54个不同的限制性内切核酸酶消化图谱（REDP）。系统发育分析无法根据地理起源对REDP进行聚类。所有与流行病学相关的分离株均显示出相同或≥91％的相似REDP。我们的研究结果表明，意大利北部的抗生素耐药性，遗传无关菌株的特殊循环。

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