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首页> 外文期刊>Journal of Clinical Microbiology >Measurement of Human Cytomegalovirus Loads by Quantitative Real-Time PCR for Monitoring Clinical Intervention in Transplant Recipients
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Measurement of Human Cytomegalovirus Loads by Quantitative Real-Time PCR for Monitoring Clinical Intervention in Transplant Recipients

机译：通过定量实时PCR测量人类巨细胞病毒载量以监测移植受者的临床干预

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Quantitative monitoring of human cytomegalovirus (HCMV) infection is helpful in determining appropriate antiviral management of transplant recipients. Quantitative PCR technologies have demonstrated accuracy in measuring systemic HCMV loads. A total of 298 consecutive whole-blood specimens submitted to the Clinical Virology Laboratory at Vanderbilt University Medical Center from 15 February to 31 October 1999 were included in the study. In addition to a qualitative colorimetric microtiter plate PCR assay (MTP-PCR) and a semiquantitative pp65 antigenemia assay, each specimen was measured for HCMV loads by a quantitative PCR assay performed on an ABI PRISM 7700 Sequence Detection System (TaqMan). Compared to results of the MTP-PCR, the sensitivity, specificity, positive predictive value, and negative predictive value were 70.5, 97.5, 87.8, and 92.8% for the antigenemia assay and were 96.7, 92.0, 75.6, and 99.1% for the TaqMan assay, respectively. There was a high correlation between antigenemia values and HCMV loads as determined by the TaqMan (r = 0.989; P < 0.001). Antigenemia values of 0, 1 to 10, 11 to 100, 101 to 1,000, and over 1,000 positive cells per 2 × 105 leukocytes corresponded to median HCMV loads measured by TaqMan of 125, 1,593, 5,713, 16,825, and 5,425,000 copies/ml, respectively. Corresponding to antigenemia values of 1 to 2, 10, and 50 positive cells per 2 × 105 leukocytes, HCMV viral loads of 1,000, 4,000, and 10,000 copies/ml are proposed as cutoff points for initiating antiviral therapy in patient groups with high, intermediate, and low risk of CMV diseases.
机译：定量监测人类巨细胞病毒（HCMV）感染有助于确定适当的移植受者抗病毒治疗。定量PCR技术已证明在测量系统HCMV负荷方面的准确性。从1999年2月15日至10月31日向范德比尔特大学医学中心临床病毒学实验室提交的总共298份连续全血标本被纳入研究。除了定性比色微量滴定板PCR测定法（MTP-PCR）和半定量pp65抗原血症测定法外，还通过在ABI PRISM 7700序列检测系统（TaqMan）上进行的定量PCR测定法测量了每个标本的HCMV载量。与MTP-PCR的结果相比，抗原血症测定的灵敏度，特异性，阳性预测值和阴性预测值分别为70.5、97.5、87.8和92.8％，而TaqMan的敏感性，特异性，阳性预测值和阴性预测值分别为96.7、92.0、75.6和99.1％化验。 TaqMan测定的抗原血症值和HCMV含量之间具有高度相关性（ r = 0.989; P <0.001）。每2×10 5 白细胞中的0、1至10、11至100、101至1,000和超过1,000个阳性细胞的抗原性值对应于TaqMan测量的HCMV中位数，分别为125、1,593、5,713，每毫升分别为16,825和5,425,000拷贝。对应于每2×10 5 白细胞1-2、10和50个阳性细胞的抗原血症值，建议以1,000、4,000和10,000拷贝/ ml的HCMV病毒载量作为开始抗病毒的临界点高，中，低CMV疾病风险患者组中的治疗。

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