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首页> 外文期刊>Journal of Clinical Microbiology >PCR-Based Genotyping of Mycobacterium tuberculosis with New GC-Rich Repeated Sequences and IS6110 Inverted Repeats Used as Primers
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PCR-Based Genotyping of Mycobacterium tuberculosis with New GC-Rich Repeated Sequences and IS6110 Inverted Repeats Used as Primers

机译:基于PCR的结核分枝杆菌基因新基因分型,具有丰富的GC重复序列和IS6110反向重复序列作为引物

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In the present study we attempted to develop a PCR-based epidemiological tool for the differentiation of Mycobacterium tuberculosis isolates. Use of the designed primers Mtb1 (5′-CCG-GCG-GGG-CCG-GCG-G) and Mtb2 (5′-CGG-CGG-CAA-CGG-CGG-C) targeting frequently repeated 16-bp sequences in combination with primers sited at the inverted repeats flanking IS6110 allowed differentiation of M. tuberculosis isolates.
机译:在本研究中,我们试图开发一种基于PCR的流行病学工具来区分结核分枝杆菌。设计的引物Mtb1(5'-CCG-GCG-GGG-CCG-GCG-G)和Mtb2(5'-CGG-CGG-CAA-CGG-CGG-C)与频繁重复的16 bp序列结合使用位于IS 6110 侧翼的反向重复序列上的引物允许 M的分化。结核病分离株。

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