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首页> 外文期刊>Journal of Clinical Microbiology >Use of Specific rRNA Oligonucleotide Probes for Microscopic Detection of Mycobacterium avium Complex Organisms in Tissue
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Use of Specific rRNA Oligonucleotide Probes for Microscopic Detection of Mycobacterium avium Complex Organisms in Tissue

机译:特异性rRNA寡核苷酸探针在组织中鸟分枝杆菌复杂生物的显微镜检测中的应用

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Members of the Mycobacterium avium complex (MAC) are important environmental pathogens that are implicated in several chronic, idiopathic diseases. Diagnosis of MAC-based diseases is compromised by the need to cultivate these fastidious and slowly growing organisms in order to identify which mycobacterial species are present. Detection is particularly difficult when MAC is intracellular or embedded within mammalian tissues. We report on the development of culture-independent, in situ hybridization (ISH) assays for the detection of MAC in culture, sputum, and tissue. This assay includes a highly reliable technique for the permeabilization of mycobacterial cells within culture and tissues. We describe a set of rRNA-based oligonucleotide probes that specifically detect either M. intracellulare, the two M. avium subspecies associated with human disease, or all members of MAC. The results call into question the validity of ISH results derived by the use of other gene loci, such as IS900.
机译:鸟分枝杆菌复合物(MAC)的成员是重要的环境病原体,与几种慢性特发性疾病有关。基于MAC的疾病的诊断因需要培养这些精疲力竭且缓慢生长的生物,以鉴定存在哪些分枝杆菌种类而受到影响。当MAC位于细胞内或嵌入哺乳动物组织内时,检测尤其困难。我们报告了文化,痰和组织中的MAC检测独立于文化,原位杂交(ISH)分析的发展。该测定法包括一种高度可靠的技术,用于使分枝杆菌细胞在培养物和组织中通透。我们描述了一组基于rRNA的寡核苷酸探针,可特异性检测任一 M。细胞内,两个 M。与人类疾病或MAC的所有成员相关的鸟亚种。结果质疑了使用其他基因位点(例如IS 900 )获得的ISH结果的有效性。

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