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首页> 外文期刊>Journal of Clinical Microbiology >Human Papillomavirus Type 16 Status in Cervical Carcinoma Cell DNA Assayed by Multiplex PCR
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Human Papillomavirus Type 16 Status in Cervical Carcinoma Cell DNA Assayed by Multiplex PCR

机译：多重PCR检测宫颈癌细胞DNA中人乳头瘤病毒16型的状态

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Integration of human papillomavirus (HPV) DNA into host genome occurs early in cancer development and is probably an important event in malignant transformation of cervical cancer. The HPV genome integration usually disrupts E2 gene open reading frames. It results in the lack of E2 gene suppressor of the synthesis of E6 and E7 products which, in turn, leads to the overexpression of E6 and E7 genes. The oncogenic HPV types (HPV16, -18, -45, and -58) can be present as episomes or may integrate into human chromosomes. Sixty-six cervical cancer patients positive for HPV16 were tested for the presence of E6, E2, E1, and L1 genes. Multiplex PCR was carried out in all cases. Using cluster analysis, the calculated ratios of E1/E6, E2/E6, L1/E6, E1/E2, and E2/(E1*E6) gene amplification products were divided into two or three statistically different groups. These were used for statistical analysis of the prevalence of specific gene types in histological types of cancer, different levels of clinical staging, and histologically confirmed nodal metastases. The statistical analysis proved a significant correlation in the ratios of E2/E6 and E1/E2 only. The E2/E6 and E1/E2 were higher in carcinoma in situ than in advanced squamous cancers. The E2/E6 ratios were lower in higher clinical stages. The multiplex PCR estimation of the E2/E6 ratio could be a simple method for selecting patients with a high risk of a poor outcome in a standard stage-dependent treatment procedure.
机译：人类乳头瘤病毒（HPV）DNA整合入宿主基因组发生在癌症发展的早期，可能是子宫颈癌恶性转化的重要事件。 HPV基因组整合通常会破坏 E2 基因开放阅读框。这导致缺乏合成E6和E7产物的 E2 基因抑制剂，进而导致 E6 和 E7 基因。致癌HPV类型（HPV16，-18，-45和-58）可以作为附加体存在，也可以整合到人类染色体中。测试了66例HPV16阳性宫颈癌患者中是否存在 E6 ， E2 ， E1 和 L1 基因。在所有情况下均进行多重PCR。使用聚类分析，计算出 E1 / E6 ， E2 / E6 ， L1 / E6 ， E1 / E2 和 E2 / （ E1 * E6 ）基因扩增产物分为两个或三个统计学上不同的组。这些用于统计学分析特定基因类型在癌症的组织学类型中的患病率，不同的临床分期水平以及组织学上已证实的淋巴结转移。统计分析表明，仅 E2 / E6 和 E1 / E2 的比率具有显着相关性。在原位癌中， E2 / E6 和 E1 / E2 高于晚期鳞状癌。在较高的临床阶段， E2 / E6 比率较低。 E2 / E6 比率的多重PCR估计可能是一种选择标准阶段依赖治疗程序中具有不良结果高风险的高风险患者的简单方法。

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