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首页> 外文期刊>Journal of Clinical Microbiology >Description of a New Yeast Species, Malassezia japonica, and Its Detection in Patients with Atopic Dermatitis and Healthy Subjects
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Description of a New Yeast Species, Malassezia japonica, and Its Detection in Patients with Atopic Dermatitis and Healthy Subjects

机译：一种新的酵母物种日本马拉丝霉的描述及其在特应性皮炎和健康受试者中的检测

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摘要

Lipophilic yeasts of the genus Malassezia are part of the normal cutaneous microflora and are considered one of the factors that trigger atopic dermatitis (AD). We isolated two strains of Malassezia from a healthy Japanese female. Analysis of the D1/D2 26S ribosomal DNA and internal transcribed spacer region sequences of the isolates suggested that they are new members of the genus Malassezia. We propose the name Malassezia japonica sp. nov. for the isolates. M. japonica is easily distinguished from the seven known lipophilic species by its ability to assimilate Tween 40 and Tween 60 and its inability to assimilate Tween 20 and Tween 80 and to grow at 40°C. Furthermore, by applying transparent dressings to the skin lesions of 36 patients with AD and the skin of 22 healthy subjects, M. japonica DNA was detected by a non-culture-based method consisting of nested PCR with M. japonica species-specific primers. M. japonica DNA was detected from 12 of the 36 patients (33.3%) and 3 of the 22 healthy subjects (13.6%). Although it is not known whether M. japonica plays a role in AD, this species was part of the microflora in both patients with AD and healthy subjects.
机译：马拉色氏菌属（Malassezia ）的亲脂酵母是正常皮肤微生物区系的一部分，被认为是诱发特应性皮炎（AD）的因素之一。我们从一名健康的日本女性中分离出了两种菌株。对分离株的D1 / D2 26S核糖体DNA和内部转录的间隔区序列的分析表明，它们是 Malassezia 属的新成员。我们建议使用名称​​ Malassezia japonica sp。十一月用于分离株。 M 。 japonica 很容易与七个已知的亲脂性物种区分开，因为它具有吸收Tween 40和Tween 60的能力，并且不能吸收Tween 20和Tween 80并在40°C下生长。此外，通过对36名AD患者的皮肤病变和22名健康受试者的皮肤使用透明敷料， M 。采用基于非培养方法的 japonica DNA进行检测，该方法由巢式PCR和 M 组成。 japonica 物种特异性引物。 M 。在36名患者中的12名（33.3％）和22名健康受试者中的3名（13.6％）中检测到了 japonica DNA。尽管还不知道 M 。 japonica 在AD中起作用，在AD患者和健康受试者中，该物种都是微生物区系的一部分。

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