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首页> 外文期刊>Journal of Clinical Microbiology >Impact of Proficiency Testing on Results of the Microscopic Agglutination Test for Diagnosis of Leptospirosis
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Impact of Proficiency Testing on Results of the Microscopic Agglutination Test for Diagnosis of Leptospirosis

机译：能力验证对镜检诊断钩端螺旋体病的影响

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A proficiency testing scheme for the leptospirosis microscopic agglutination test was provided to 37 laboratories in 23 countries in 2002 (round 1) and to 60 laboratories in 34 countries in 2003 (round 2). Thirty-four laboratories participated in both rounds. Each panel consisted of five rabbit serum samples, four of which were antisera raised against pathogenic serovars of Leptospira. One of these samples was a mixture of two different antisera. The rates of false-negative results, calculated on the basis of the assumption that serovars within a serogroup will cross-react, were 11% for round 1 and 14% for round 2. There were regional differences in the rates of false-negative results. The titers reported by laboratories testing for the same sample with the same serovar varied widely. Laboratories that had previously participated in round 1 reported fewer false-negative results in round 2 than new participants (10 and 21%, respectively [P = 0.002]) and reported 0.56 false-negative results per participant, whereas new participants reported 1.23 false-negative results per participant (P = 0.041). Laboratories that had previously participated also reported fewer false-negative results in round 2 than in round 1 when samples common to both rounds were tested (5 and 15%, respectively [P = 0.028]). The titers reported by the new participants were, on average, lower than those reported by the laboratories that had participated previously (P = 0.019) and were significantly more variable (P = 0.001). Analysis of these results suggests a positive impact of proficiency testing on the testing performance of the participating laboratories.
机译：在2002年（第1轮）向23个国家的37个实验室（2003年）提供了一个钩端螺旋体显微镜凝集测试能力验证计划，在2003年（第2轮）向34个国家的60个实验室提供了一个能力验证计划。两个回合中有34个实验室参加。每个小组由五只兔血清样品组成，其中四份是针对 Leptospira 的致病血清的抗血清。这些样品之一是两种不同抗血清的混合物。假阴性结果的发生率是基于血清群中血清型会交叉反应的假设计算得出的，第1轮为11％，第2轮为14％。假阴性结果的比率存在区域差异。实验室报告的具有相同血清型的相同样品的滴度差异很大。先前参与第1轮的实验室报告的第2轮假阴性结果少于新参与者（分别为10％和21％[ P = 0.002]），每名参与者报告的假阴性结果为0.56，而新参与者报告的每位参与者的假阴性结果为1.23（ P = 0.041）。以前参加过实验的实验室在测试第二轮相同的样本时，第二轮的假阴性结果也比第一轮少（分别为5％和15％[ P = 0.028]）。新参与者报告的滴度平均低于先前参与实验室的报告的滴度（ P = 0.019），并且变化更大（ P = 0.001）。对这些结果的分析表明，水平测试对参与实验室的测试性能具有积极影响。

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