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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of Enzyme-Linked Immunosorbent Assays, an Immunofluorescent-Antibody Test, and Two Rapid Tests (Immunochromatographic-Dipstick and Gel Tests) for Serological Diagnosis of Symptomatic and Asymptomatic Leishmania Infections in Dogs
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Evaluation of Enzyme-Linked Immunosorbent Assays, an Immunofluorescent-Antibody Test, and Two Rapid Tests (Immunochromatographic-Dipstick and Gel Tests) for Serological Diagnosis of Symptomatic and Asymptomatic Leishmania Infections in Dogs

机译：评价酶联免疫吸附试验，免疫荧光抗体试验和两种快速试验（免疫色谱试纸和凝胶试验），用于狗有症状和无症状利什曼原虫感染的血清学诊断

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Enzyme-linked immunosorbent assays (ELISAs) based on soluble antigens derived from promastigote or amastigote-like stages of Leishmania infantum and on the recombinant rK39 antigen, each in combination with different conjugates [anti-immunoglobulin G1 [IgG1], anti-IgG2, anti-IgG(γ), and anti-IgG heavy plus light chains], were compared to an immunofluorescent-antibody test (IFAT) and two commercially available rapid test systems (DiaMed-Vet-IT Leish and ID-PaGIA canine leishmaniasis antibody test) for the detection of specific anti-Leishmania antibodies in symptomatic and asymptomatic dogs with proven L. infantum infections. ELISAs based on soluble promastigote and amastigote antigens had very high sensitivities in symptomatic (n = 30; 100%) and asymptomatic dogs (n = 17; 94.1 to 100%), except when combined with the anti-IgG1 conjugate (41.2 to 82.4%). Specificities were high for all combinations (n = 50; 96 to 100%). The rK39 ELISA detected fewer asymptomatic cases (sensitivities, 52.9 to 64.7%) but was highly specific (96 to 100%). The IFAT was 90% sensitive in symptomatic dogs but was significantly less sensitive in asymptomatic cases (29.4%). However, it had an excellent specificity (100%). Test performances of the rapid tests based on the rK39 antigen were comparable to the ELISAs based on the same antigen. ELISAs based on soluble promastigote or amastigote antigens seem to be most suited for the serological diagnosis of canine Leishmania infections in both symptomatic and asymptomatic dogs. IFAT and the rK39 ELISA lack sensitivity in asymptomatic cases but are highly specific. Rapid tests like the rK39 dipstick test or the ID-PaGIA are helpful for confirming clinically suspected cases because of their high specificities in symptomatic animals.
机译：酶联免疫吸附测定（ELISA），其基于源自婴儿利什曼原虫的前鞭毛体或类似鞭毛体的阶段的可溶性抗原和重组rK39抗原，每种结合有不同的结合物[抗免疫球蛋白G1 [IgG1 ]，抗IgG2，抗IgG（γ）和抗IgG重链和轻链]，免疫荧光抗体测试（IFAT）和两种市售快速测试系统（DiaMed-Vet-IT Leish和ID -PaGIA犬利什曼病抗体测试）可用于检测具有确诊的 L的有症状和无症状狗的特异性抗 Leishmania 抗体。婴儿感染。基于可溶性前鞭毛体和鞭毛体抗原的ELISA对有症状（ n = 30; 100％）和无症状犬（ n = 17; 94.1至100％）的敏感性很高，与抗IgG1偶联物组合时除外（41.2％至82.4％）。所有组合的特异性都很高（ n = 50; 96至100％）。 rK39 ELISA检测出较少的无症状病例（敏感性为52.9至64.7％），但特异性很高（96至100％）。 IFAT在有症状的狗中敏感度为90％，但在无症状的情况下敏感度明显较低（29.4％）。但是，它具有出色的特异性（100％）。基于rK39抗原的快速测试的测试性能与基于相同抗原的ELISA相当。基于可溶性前鞭毛体或鞭毛体抗原的ELISA似乎最适合有症状和无症状犬的血清 Leishmania 感染的血清学诊断。 IFAT和rK39 ELISA在无症状的情况下缺乏敏感性，但具有高度特异性。 rK39量油尺测试或ID-PaGIA等快速测试对有症状的动物具有高度特异性，因此有助于确诊临床可疑病例。

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