...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Use of Pooled Urine Samples and Automated DNA Isolation To Achieve Improved Sensitivity and Cost-Effectiveness of Large-Scale Testing for Chlamydia trachomatis in Pregnant Women
【24h】

Use of Pooled Urine Samples and Automated DNA Isolation To Achieve Improved Sensitivity and Cost-Effectiveness of Large-Scale Testing for Chlamydia trachomatis in Pregnant Women

机译：使用合并的尿液样本和自动DNA分离来提高孕妇沙眼衣原体大规模检测的灵敏度和成本效益

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The success of large-scale screening for Chlamydia trachomatis depends on the availability of noninvasive samples, low costs, and high-quality testing. To evaluate C. trachomatis testing with pregnant women, first-void urine specimens from 750 consecutive asymptomatic pregnant women from the Rotterdam area (The Netherlands) were collected. Initially, we investigated the performance of three different DNA isolation methods with 350 of these urines and 70 pools of 5 of the same subset of urine samples. The routinely used COBAS AMPLICOR test was compared to the COBAS AMPLICOR test with prior DNA isolation by use of the MagNA Pure large-volume kit and the MagNA Pure bacterial DNA isolation kit. The latter combination provided the best DNA test for pooled urines, with a sensitivity twice that of the other methods. Next, using all 750 urines, the COBAS AMPLICOR performance for individual testing was compared to pooled testing with the standard COBAS AMPLICOR procedure and subsequently to pooled testing with COBAS AMPLICOR in combination with the MagNA Pure bacterial DNA isolation kit. The sensitivity of COBAS AMPLICOR was 65% on individual and 42% on pooled urines but improved to 92% on pooled urines with the MagNA Pure bacterial DNA isolation kit, making this combination the best screening method. The C. trachomatis prevalence in this population appeared to be 6.4%. Additionally, the cost of the combined MagNA Pure bacterial DNA isolation kit and COBAS AMPLICOR method on pooled urines was only 56% of the cost of the standard COBAS AMPLICOR test applied to individual urines. Costs per positive case detected in the combined method were 39% of standard costs.
机译：沙眼衣原体大规模筛查的成功与否取决于无创样本的可获得性，低成本和高质量检测。评估 C。对孕妇进行沙眼衣原体检测，收集了来自荷兰鹿特丹地区的750名连续无症状孕妇的初次尿样。最初，我们研究了三种不同的DNA分离方法对350种尿液和70个合并的5个相同尿液样本子集的性能。通过使用MagNA Pure大容量试剂盒和MagNA Pure细菌DNA分离试剂盒，将常规使用的COBAS AMPLICOR测试与事先进行DNA分离的COBAS AMPLICOR测试进行了比较。后一种组合为合并的尿液提供了最佳的DNA测试，其灵敏度是其他方法的两倍。接下来，使用全部750尿液，将个人测试的COBAS AMPLICOR性能与标准COBAS AMPLICOR程序的合并测试进行比较，随后与COBAS AMPLICOR结合MagNA Pure细菌DNA分离试剂盒进行合并测试进行比较。使用MagNA Pure细菌DNA分离试剂盒，COBAS AMPLICOR对个体的敏感性为65％，对合并尿液的敏感性为42％，但对合并尿液的敏感性提高至92％，使该组合成为最佳筛选方法。 C。该人群中沙眼的患病率为6.4％。此外，合并的MagNA Pure细菌DNA分离试剂盒和COBAS AMPLICOR方法在合并尿液中的成本仅为标准COBAS AMPLICOR测试在单个尿液中的成本的56％。合并方法中发现的每例阳性病例的成本为标准成本的39％。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号