Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis

G. B. Rogers; M. P. Carroll; D. J. Serisier; P. M. Hockey; G. Jones; V. Kehagia; G. J. Connett; K. D. Bruce

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Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis

机译：通过终端限制性片段长度多态性分析对16S rRNA基因谱进行分析，以比较囊性纤维化患者痰液和漱口液中的细菌群落

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The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found.

机译：通过使用从临床样品中直接从细菌中提取的核酸扩增得到的16S rRNA基因PCR产物的末端限制性片段长度多态性分析，对19名成人囊性纤维化（CF）患者口腔和肺中存在的细菌群落进行了比较。痰液样本未发现受到口腔细菌的严重污染。已发现某些口腔细菌物种使CF肺定殖的证据。

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《Journal of Clinical Microbiology》 |2006年第7期|共4页
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G. B. Rogers; M. P. Carroll; D. J. Serisier; P. M. Hockey; G. Jones; V. Kehagia; G. J. Connett; K. D. Bruce;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis [J] . G. B. Rogers, M. P. Carroll, D. J. Serisier, Journal of Clinical Microbiology . 2006,第7期

机译：通过终端限制性片段长度多态性分析对16S rRNA基因谱进行分析，以比较囊性纤维化患者痰液和漱口液中的细菌群落
2. Bacterial Diversity in Cases of Lung Infection in Cystic Fibrosis Patients: 16S Ribosomal DNA (rDNA) Length Heterogeneity PCR and 16S rDNA Terminal Restriction Fragment Length Polymorphism Profiling [J] . G. B. Rogers, C. A. Hart, J. R. Mason, Journal of Clinical Microbiology . 2003,第8期

机译：囊性纤维化患者肺部感染的细菌多样性：16S核糖体DNA（rDNA）长度异质性PCR和16S rDNA末端限制性片段长度多态性分析
3. Characterization of Bacterial Community Diversity in Cystic Fibrosis Lung Infections by Use of 16S Ribosomal DNA Terminal Restriction Fragment Length Polymorphism Profiling [J] . G. B. Rogers, M. P. Carroll, D. J. Serisier, Journal of Clinical Microbiology . 2004,第11期

机译：囊性纤维化肺部感染细菌群落多样性的表征通过使用16S核糖体DNA终端限制片段长度多态性分析。
4. Microbial Community Structure of Riparian Zone in wenyu River Analysis by Terminal-Restriction Fragment Length Polymorphisms [C] . Ping DONG, Yujiao SUN, Hongqi WANG International conference on environment simulation and pollution control . 2011

机译：终端限制片段长度多态性分析温榆河河岸带微生物群落结构
5. Qualitative assessments and computational techniques for the studies of microbial diversity based on terminal restriction fragment length polymorphism (T-RFLP) of 16S and 18S rRNA gene sequences [D] . Shyu, Conrad. 2006

机译：基于16S和18S rRNA基因序列的末端限制性片段长度多态性（T-RFLP）的微生物多样性研究的定性评估和计算技术
6. Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis [O] . G. B. Rogers, M. P. Carroll, D. J. Serisier, 2006

机译：通过终端限制性片段长度多态性分析使用16S rRNA基因谱分析比较囊性纤维化患者痰液和漱口液中的细菌群落
7. Use of 16S rRNA gene profiling by terminal restriction fragment length polymorphism analysis to compare bacterial communities in sputum and mouthwash samples from patients with cystic fibrosis [O] . Rogers G. B., Carroll M. P., Serisier D. J., 2006

机译：使用16S rRNA基因图谱进行末端限制性片段长度多态性分析，比较囊性纤维化患者痰液和漱口水样品中的细菌群落

Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis

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