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首页> 外文期刊>Journal of Clinical Microbiology >Effect of endocervical specimen quality on detection of Chlamydia trachomatis and on the incidence of false-positive results with the Chlamydiazyme method.
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Effect of endocervical specimen quality on detection of Chlamydia trachomatis and on the incidence of false-positive results with the Chlamydiazyme method.

机译：宫颈内标本质量对沙眼衣原体检测和衣原体酶法假阳性结果发生率的影响。

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Duplicate endocervical swabs were collected from 1,675 patients to assess the effects of variations in specimen quality on Chlamydiazyme (Abbott Laboratories) detection of Chlamydia trachomatis and the incidence of false-positive results. One swab (at random) from each patient was tested for C. trachomatis antigen by using the standard Chlamydiazyme procedure. A 200-microliter volume of 0.9% saline was added to the other swab from each patient. After vortexing, 20 microliters was smeared on a slide for Papanicolaou (Pap) staining and the remaining specimen was then tested with the Chlamydiazyme assay. The Chlamydiazyme result was positive for 170 (10.1%) and 165 (9.8%) of the stained and unstained duplicate specimens, respectively (no significant difference). Pap stains on smears from 1,536 (91.7%) of the patients were analyzed, and endocervical and/or metaplastic (E-M) cells were detected in 789 (51.4%) smears. Of these 1,536 stained and analyzed specimens, 150 (9.8%) were Chlamydiazyme positive but only 132 (88.0%) of the positive results were confirmed by repeating the test and using a monoclonal blocking antibody (Abbott). Confirmed Chlamydiazyme-positive results were obtained from only 34 (4.6%) of 747 specimens lacking E-M cells but from 98 (12.4%) of 789 specimens containing the cells (P less than 0.001). Of the 150 initially Chlamydiazyme-positive results obtained with Pap-stained, analyzed specimens, 12 (26.1%) of 46 were falsely positive from specimens lacking E-M cells but only 6 (5.8%) of 104 were falsely positive from specimens containing E-M cells (P less than 0.01). C. trachomatis antigen was detected significantly more frequently and false-positive results were significantly less common from specimens in which E-M cells were detected.
机译：从1,675名患者中收集了重复的宫颈拭子，以评估标本质量变化对沙眼衣原体（沙眼衣原体）检测和假阳性结果发生率的影响。通过使用标准衣原体双酶程序，对每位患者的一支拭子（随机）进行了沙眼衣原体抗原的检测。向每位患者的另一支拭子中加入200微升0.9％的盐水。涡旋后，将20微升涂在载玻片上进行帕潘尼古拉（Pap）染色，然后用衣原体酶测定法测试其余标本。染色质和未染色重复标本的衣原体酶结果分别为170（10.1％）和165（9.8％）阳性（无显着性差异）。分析了1,536（91.7％）例患者涂片上的子宫颈抹片染色，并在789例（51.4％）涂片中检出了宫颈和/或化生（E-M）细胞。在这1,536份染色和分析的标本中，衣原体酶为150（9.8％）阳性，但是通过重复测试并使用单克隆封闭抗体（Abbott）可以确认阳性结果中只有132（88.0％）。从747个缺少E-M细胞的标本中只有34个（4.6％）获得了衣原体酶阳性结果，但从789个含有E-M细胞的标本中有98个（12.4％）获得了证实的衣原体阳性结果（P小于0.001）。在用巴氏染色的分析标本中获得的150例最初的衣原体酶阳性结果中，有46个中有12个（26.1％）从缺乏EM细胞的标本中呈假阳性，但在104个中只有6个（5.8％）从含有EM细胞的标本中呈假阳性（ P小于0.01）。在检测到E-M细胞的标本中，沙眼衣原体抗原的检出率更高，假阳性结果的发生率也更低。

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