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首页> 外文期刊>Journal of Clinical Microbiology >Restriction fragment analysis of a Candida tropicalis outbreak of sternal wound infections.
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Restriction fragment analysis of a Candida tropicalis outbreak of sternal wound infections.

机译：热带假丝酵母胸骨伤口感染暴发的限制性片段分析。

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摘要

An apparent single-source outbreak of Candida tropicalis sternal wound infections in eight patients was investigated by utilizing DNA restriction fragment analysis (RFA) with HindIII and BstNI. All eight outbreak isolates appeared to be identical and were easily differentiated from control isolates by DNA RFA. Compared with an arbitrarily selected reference outbreak isolate, greater than or equal to 95% of the bands in the restriction digests identified by a computerized image analysis system from each of the outbreak isolates were identical versus 13 to 53% of the bands in any of the nine control isolates. Outbreak strains were significantly more likely to match the reference outbreak isolate than were controls (P less than 0.0001). The RFA was greatly facilitated by the use of computerized image analysis and confirmed the epidemiologic link between a scrub nurse and the infected patients.
机译：利用HindIII和BstNI进行的DNA限制性片段分析（RFA），调查了八例患者中明显的热带假丝酵母胸骨伤口感染的单源暴发。所有八个暴发分离株似乎都是相同的，并且很容易通过DNA RFA与对照分离株区分开。与任意选择的参考暴发隔离株相比，通过计算机图像分析系统从每种暴发分离株中鉴定的限制性酶切消化带中，大于或等于95％的谱带相同，而在任何一种暴发分离株中，这些谱带中的13％至53％相同九个对照菌株。与对照相比，暴发菌株更可能与参考暴发菌株相匹配（P小于0.0001）。通过使用计算机图像分析极大地促进了RFA，并证实了擦洗护士和受感染患者之间的流行病学联系。

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