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首页> 外文期刊>Journal of Clinical Microbiology >Beta-glucuronidase activities of fecal isolates from healthy swine.
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Beta-glucuronidase activities of fecal isolates from healthy swine.

机译：来自健康猪的粪便分离物的β-葡萄糖醛酸酶活性。

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Research has shown that various percentages of fecal Escherichia coli isolates obtained from healthy subjects may be beta-glucuronidase negative. The ability to detect beta-glucuronidase activity among fecal E. coli isolates from healthy subjects may be affected by assay conditions. A study was conducted in which agar and broth media containing 4-methylumbelliferyl-beta-D-glucuronide (MUG) were used to examine beta-glucuronidase activities of fecal isolates from healthy swine. Rectal swabs were plated on MacConkey agar plus 100 mg of MUG per liter (MAC-MUG) and incubated at 35 degrees C for 24 h. Each of 986 isolates picked from MAC-MUG was inoculated into duplicate tubes of lauryl tryptose broth plus 50 mg of MUG per liter (LT-MUG). One set of tubes was incubated at 35 degrees C and the other set of tubes was incubated at 44.5 degrees C. Gas production and hydrolysis of MUG, indicated by fluorescence when observed with UV light with a wavelength of 360 nm, were determined after incubation for 24 and 48 h. A higher percentage (P less than 0.01) of isolates was MUG positive at 44.5 degrees C than at 35 degrees C after 24 h of incubation in LT-MUG. A higher percentage (P less than 0.01) of isolates was MUG positive after 48 h than after 24 h of incubation at both 35 and 44.5 degrees C. A lower percentage of isolates (P less than 0.05) was observed to be MUG positive on MAC-MUG agar compared with their MUG reactions in LT-MUG at 35 and 44.5 degrees C. Approximately 89% of the isolates identified were beta-glucuronidase-positive E. coli. The largest proportion of MUG-positive E. coli was detected with LT-MUG at 35 degrees C after 48 h of incubation.
机译：研究表明，从健康受试者中获得的不同百分比的粪便大肠杆菌分离物可能是β-葡萄糖醛酸苷酶阴性的。检测条件会影响检测健康受试者粪便大肠杆菌分离物中β-葡萄糖醛酸苷酶活性的能力。进行了一项研究，其中使用含有4-甲基伞形酮基-β-D-葡糖醛酸苷（MUG）的琼脂和肉汤培养基检查健康猪粪中分离物的β-葡糖醛酸苷酶活性。将直肠拭子涂在每升含100 mg MUG（MAC-MUG）的MacConkey琼脂上，并在35摄氏度下孵育24小时。从MAC-MUG中提取的986株分离株中的每一个都接种到重复的月桂基胰蛋白bro肉汤管中，每升含50 mg MUG（LT-MUG）。一组试管在35摄氏度下孵育，另一组试管在44.5摄氏度下孵育。孵育30分钟后，测定了MUG的产气量和水解度，当用波长为360 nm的紫外线观察时，荧光表示24和48小时。在LT-MUG中孵育24小时后，在44.5°C时，分离株的MUG阳性率更高（P小于0.01），而在35°C时则呈阳性。在35和44.5摄氏度下孵育48小时后，比24小时孵育后更高的分离物（P小于0.01）呈MUG阳性。在MAC上观察到更低的分离物（P小于0.05）呈MUG阳性-MUG琼脂与其在LT-MUG中在35和44.5摄氏度下的MUG反应相比。鉴定出的分离株中约89％为β-葡萄糖醛酸苷酶阳性大肠杆菌。孵育48小时后，在35摄氏度下用LT-MUG检测到最大比例的MUG阳性大肠杆菌。

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