VP4 typing of bovine and porcine group A rotaviruses by PCR.

V Gouvea; N Santos; M do C Timenetsky

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VP4 typing of bovine and porcine group A rotaviruses by PCR.

机译：通过PCR对牛和猪A群轮状病毒进行VP4分型。

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A PCR typing assay was developed to identify rotavirus P types (VP4 specificity) of bovine NCDV, UK, and B223 strains and porcine OSU and Gottfried strains. Thirty-nine human and animal strains representing all known, and some undefined, rotavirus P types were used to develop and evaluate the specificity of the method. No cross-amplification was observed. The PCR results agreed with previous characterizations by monoclonal antibodies, sequence analysis, and hybridization assays, except for the Gottfried strain, which showed a P type distinct from the human asymptomatic strains. Analysis of a small number of field specimens suggested a high level of VP4 polymorphism among porcine strains. The assay should be of value in typing field isolates and tracing interspecies infections.

机译：开发了一种PCR分型测定法，以鉴定牛NCDV，UK和B223株以及猪OSU和Gottfried株的轮状病毒P型（VP4特异性）。使用代表所有已知的和一些未定义的轮状病毒P型的三十九种人和动物株来开发和评估该方法的特异性。没有观察到交叉扩增。 PCR结果与以前通过单克隆抗体，序列分析和杂交测定所表征的特征相吻合，除了Gottfried菌株，该菌株显示出不同于人类无症状菌株的P型。对少量野外标本的分析表明，猪株中VP4多态性较高。该测定法应在键入田间分离株和追踪种间感染方面具有价值。

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《Journal of Clinical Microbiology》 |1994年第5期|共5页
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V Gouvea; N Santos; M do C Timenetsky;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Sequence analysis of the VP7 and VP4 genes identifies a novel VP7 gene allele of porcine rotaviruses, sharing a common evolutionary origin with human G2 rotaviruses [J] . Martella V, Ciarlet M, Baselga R, Virology . 2005,第1期

机译：VP7和VP4基因的序列分析确定了猪轮状病毒的新VP7基因等位基因，与人类G2轮状病毒具有共同的进化起源
2. Development of cDNA probes for typing group A bovine rotaviruses on the basis of VP4 specificity. [J] . A V Parwani, B I Rosen, M A McCrae, Journal of Clinical Microbiology . 1992,第10期

机译：基于VP4特异性的A型牛轮状病毒分型cDNA探针的开发。
3. Molecular characterization of the VP1, VP2, VP4, VP6, NSP1 and NSP2 genes of bovine group B rotaviruses: identification of a novel VP4 genotype. [J] . Ghosh S, Kobayashi N, Nagashima S, Archives of virology . 2010,第2期

机译：牛B型轮状病毒VP1，VP2，VP4，VP6，NSP1和NSP2基因的分子表征：鉴定新的VP4基因型。
4. Incidence of porcine coronaviruses and porcine rotaviruses in an Indiana swine operation following clinical onset of acute diarrhea [C] . C. Schnur, D. Ragland, R. Pogranichniy Annual^Meeting of the American^Association^of^Swine^Veterinarians. . 2016

机译：在临床发作后急性腹泻的临床发作后印第安纳猪手术中猪冠状病毒和猪旋转血管的发病率
5. Characterization of the P and G types and the VP4 and VP7 gene divergence among group A bovine rotaviruses. [D] . Mummidi, Srinivas. 1996

机译：A组牛轮状病毒之间P和G类型的特征以及VP4和VP7基因的差异。
6. VP4 typing of bovine and porcine group A rotaviruses by PCR. [O] . V Gouvea, N Santos, M do C Timenetsky 1994

机译：通过PCR对牛和猪A群轮状病毒进行VP4分型。
7. VP4 typing of bovine and porcine group A rotaviruses by PCR [O] . V Gouvea, N Santos, M do C Timenetsky 1994

机译：VP4通过PCR键入牛和猪组旋转血清

VP4 typing of bovine and porcine group A rotaviruses by PCR.

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