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首页> 外文期刊>Journal of Clinical Microbiology >Murine infection model for maintenance and amplification of Cryptosporidium parvum oocysts.
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Murine infection model for maintenance and amplification of Cryptosporidium parvum oocysts.

机译：用于维持和扩增小隐孢子虫卵囊的小鼠感染模型。

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Propagation of Cryptosporidium parvum is problematic because in vitro development of the parasite is poor and animals are only briefly susceptible as neonates. At present oocysts of the parasite are usually procured by passage in neonatal sheep or cattle. In the present study, large numbers of oocysts of C. parvum could be isolated following infection of dexamethasone-treated adult C57BL/6 mice. The amount of immunosuppressive drug and the regimen of administration were critical for successful maintenance of the parasite, however. Routinely, 10 mice (age, 8 to 12 weeks) were injected four times on alternate days with 1.0 mg of dexamethasone, and the last injection was given on the same day as oral inoculation with 10(6) oocysts. By using a simplified procedure for oocyst purification from mouse feces, approximately 10(9) oocysts were obtained. This model is inexpensive and comparatively safe to handle, and the numbers of animals inoculated can be varied to obtain the required number of oocysts. Thus, this murine infection model would be a suitable alternative to the use of neonatal calves or sheep for efficient oocyst propagation.
机译：小球隐孢子虫的繁殖是有问题的，因为该寄生虫的体外发育很差，并且动物只是作为新生儿短暂易感。目前，通常通过在新生绵羊或牛中传代来获得该寄生虫的卵囊。在本研究中，感染地塞米松治疗的成年C57BL / 6小鼠后，可以分离出大量C. parvum卵囊。但是，免疫抑制药物的量和给药方案对于成功维持寄生虫至关重要。通常，每隔10天（年龄8至12周）向10只小鼠注射四次1.0毫克地塞米松，最后一次注射与口服10（6）个卵囊的同一天。通过使用简化的程序从小鼠粪便中纯化卵囊，获得了大约10（9）个卵囊。该模型价格便宜，并且处理起来相对安全，可以改变接种的动物数量以获得所需的卵囊数量。因此，这种鼠类感染模型将是使用新生儿犊牛或绵羊进行卵囊有效繁殖的合适替代方法。

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