Nonisotopic hybridization assay for determination of relative amounts of genotypic human immunodeficiency virus type 1 zidovudine resistance.

P S Eastman; E Boyer; L Mole; J Kolberg; M Urdea; M Holodniy

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Nonisotopic hybridization assay for determination of relative amounts of genotypic human immunodeficiency virus type 1 zidovudine resistance.

机译：非同位素杂交测定法，用于测定基因型人免疫缺陷病毒1型齐多夫定耐药性的相对量。

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摘要

A nonisotopic hybridization assay for human immunodeficiency virus genotypic zidovudine resistance determination is described. Biotinylated PCR product was hybridized with enzyme-labeled probes for wild-type or resistant mutant sequences and detected colorimetrically or chemiluminescently in a microplate format. Changes in mutant-to-wild-type ratios allow for the monitoring of longitudinal patient samples.

机译：描述了一种用于人类免疫缺陷病毒基因型齐多夫定耐药性测定的非同位素杂交测定法。将生物素化的PCR产物与酶标记的野生型或抗性突变序列的探针杂交，并以微孔板的形式进行比色或化学发光检测。突变体与野生型比例的变化允许监测纵向患者样品。

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《Journal of Clinical Microbiology》 |1995年第10期|共4页
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P S Eastman; E Boyer; L Mole; J Kolberg; M Urdea; M Holodniy;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Effect of zidovudine resistance mutations on virologic response to treatment with zidovudine-lamivudine-ritonavir: genotypic analysis of human immunodeficiency virus type 1 isolates from AIDS clinical trials group protocol 315.ACTG Protocol 315 Team. [J] . Kuritzkes DR, Sevin A, Young B, The Journal of Infectious Diseases . 2000,第2期

机译：齐多夫定耐药性突变对齐多夫定-拉米夫定-利托那韦治疗的病毒学应答的影响：来自人类免疫缺陷病毒1型分离株的基因型分析，来自AIDS临床试验小组协议315。ACTG协议315小组。
2. Evaluation of infection with human immunodeficiency virus type 1 by using nonisotopic solution hybridization for detection of polymerase chain reaction-amplified proviral DNA. [J] . F Coutlée, P Saint-Antoine, C Olivier, Journal of Clinical Microbiology . 1991,第11期

机译：通过使用非同位素溶液杂交检测聚合酶链反应扩增的原病毒DNA，评估1型人类免疫缺陷病毒的感染情况。
3. Alkylglycerol prodrugs of phosphonoformate are potent in vitro inhibitors of nucleoside-resistant human immunodeficiency virus type 1 and select for resistance mutations that suppress zidovudine resistance. [J] . Hammond JL, Koontz DL, Bazmi HZ, Antimicrobial agents and chemotherapy. . 2001,第6期

机译：膦酸酯的烷基甘油前药是1型核苷抗性人类免疫缺陷病毒的有效体外抑制剂，并选择可抑制齐多夫定耐药性的耐药性突变。
4. Evolution of relative synonymous codon usage in Human Immunodeficiency Virus type-1 [C] . Peter L. Meintjes, Allen G. Rodrigo Proceedings of the Second conference on Asia-Pacific bioinformatics . 2004

机译：人类免疫缺陷病毒1型相对同义密码子使用的演变
5. Treatment of human immunodeficiency virus with zidovudine and tenofovir regimen in India: A comparative effectiveness study. [D] . Thuppal, Sowmyanarayanan. 2015

机译：在印度用齐多夫定和替诺福韦方案治疗人类免疫缺陷病毒：比较效果研究。
6. Nonisotopic hybridization assay for determination of relative amounts of genotypic human immunodeficiency virus type 1 zidovudine resistance. [O] . P S Eastman, E Boyer, L Mole, 1995

机译：非同位素杂交测定法用于测定基因型人免疫缺陷病毒1型齐多夫定耐药性的相对量。
7. Nonisotopic hybridization assay for determination of relative amounts of genotypic human immunodeficiency virus type 1 zidovudine resistance [O] . P S Eastman, E Boyer, L Mole, 1995

机译：用于测定基因型人免疫缺陷病毒的相对量的非同岩杂交测定1型Zidovine抗性
8. Report on Carcinogens (RoC) Concept: Selected Viruses. Epstein--Barr Virus, Human ImmunodeficiencynVirus Type 1, Human T--Cell Lymphotropic Virus Type 1, Kaposi Sarcoma--Associated Herpes Virus, and nMerkel Cell Polyoma Virus. [R] . 2016

机译：关于致癌物（RoC）概念的报告：选定的病毒。爱泼斯坦 - 巴尔病毒，人类免疫缺陷病毒1型，人类T细胞淋巴细胞病毒1型，卡波西肉瘤 - 相关疱疹病毒和nmerkel细胞多瘤病毒。

Nonisotopic hybridization assay for determination of relative amounts of genotypic human immunodeficiency virus type 1 zidovudine resistance.

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