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首页> 外文期刊>Journal of Clinical Microbiology >Detection of Antibodies to Pasteurella multocida by capture enzyme immunoassay using a monoclonal antibody against P37 antigen.
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Detection of Antibodies to Pasteurella multocida by capture enzyme immunoassay using a monoclonal antibody against P37 antigen.

机译：使用抗P37抗原的单克隆抗体通过捕获酶免疫法检测多杀巴斯德氏菌抗体。

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As infection with Pasteurella multocida is common in rabbits, an enzyme immunoassay (EIA) was developed for its detection. A murine immunoglobulin G monoclonal antibody was used to capture a 37-kDa polypeptide of P. multocida serotype A:12 in an EIA to detect antibodies to P. multocida. The 37-kDa antigen was selected since it was previously shown to be a major immunogen during P. multocida infection in rabbits. The sensitivity of the P37 EIA, determined with sera from 56 rabbits infected with P. multocida, was 98%. Specificity, evaluated with sera from 62 rabbits from colonies free of P. multocida, was 92%. Titration curves of sera from rabbits immunized with P. multocida serotype A:3 or A:12 coincided, indicating that the P37 EIA was equally efficient in detecting antibodies to the two major serotypes of the organism. Comparison of the P37 EIA with the current serodiagnostic test, a bacterial lysate EIA, revealed relatively good correlation (r = 0.68). However, specificity was greatly improved, as 34% of uninfected rabbits were falsely positive by the lysate EIA whereas only 3% of uninfected rabbits were falsely positive by the P37 EIA. The coefficient of variation for same-day tests was 10%, and that for interday tests was 15%, indicating good reproducibility. The greater sensitivity and specificity of the P37 EIA should significantly enhance diagnostic capability to identify rabbits infected with P. multocida.
机译：由于多杀性巴氏杆菌感染在兔子中很常见，因此开发了一种酶联免疫测定法（EIA）进行检测。鼠免疫球蛋白G单克隆抗体被用来捕获EIA中多杀性疟原虫血清型A：12的37kDa多肽，以检测针对多杀性疟原虫的抗体。选择37 kDa抗原是因为先前已证明它是家兔多杀青霉感染期间的主要免疫原。用感染多杀性疟原虫的56只兔子的血清测定的P37 EIA的敏感性为98％。用来自多杀性疟原虫菌落的62只兔的血清评估的特异性为92％。 A.3或A：12多重致死性疟原虫血清免疫的兔血清的滴定曲线重合，表明P37 EIA在检测针对该生物的两种主要血清型的抗体方面同样有效。将P37 EIA与目前的血清诊断细菌裂解物EIA进行诊断比较，相关性相对较好（r = 0.68）。但是，特异性得到了极大的改善，因为34％的未感染兔子被裂解液EIA假阳性，而只有3％的未感染兔子被P37 EIA假阳性。当日测试的变异系数为10％，而当日测试的变异系数为15％，表明再现性良好。 P37 EIA具有更高的敏感性和特异性，应该会大大增强诊断感染多杀性巴氏杆菌的兔子的诊断能力。

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