Identification of Encephalitozoon intestinalis in Travelers with Chronic Diarrhea by Specific PCR Amplification

Laurent Raynaud; Frederic Delbac; Veronique Broussolle; Meja Rabodonirina; Veronique Girault; Martine Wallon; Gregoire Cozon; Christian P. Vivares; Fran?ois Peyron

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Identification of Encephalitozoon intestinalis in Travelers with Chronic Diarrhea by Specific PCR Amplification

机译：特异性PCR扩增鉴定慢性腹泻旅客脑小肠

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With the use of Weber’s modified trichrome and Uvitex 2B techniques, spores of microsporidia were detected in the stools of four travelers presenting clinically with chronic diarrhea. The general health of these patients was not impaired, and human immunodeficiency virus screening was negative. Immune evaluation, including the study of lymphocytic subpopulations, assay of serum immunoglobulins, and an intradermal multitest, showed normal results. Molecular identification of microsporidian species was based on the PCR amplification of a small-subunit rRNA sequence followed by HinfI endonuclease restriction. Encephalitozoon intestinalis microsporidiosis was thus shown in two of the four patients examined. In two patients, therapy based on albendazole made stools devoid of microsporidian spores without influence on the intestinal disorders. The pathogenic role of E. intestinalis in immunocompetent individuals remains to be demonstrated.

机译：使用韦伯改良的三色和Uvitex 2B技术，在临床上表现为慢性腹泻的四名旅行者的粪便中检测到了小孢子虫的孢子。这些患者的整体健康没有受到损害，人类免疫缺陷病毒筛查为阴性。免疫评估，包括淋巴细胞亚群的研究，血清免疫球蛋白的测定以及皮内多重测试，均显示正常结果。微孢子虫种类的分子鉴定是基于小亚基rRNA序列的PCR扩增，然后是 Hin fI核酸内切酶限制酶。因此，在所检查的四名患者中，有两名患者显示了脑肠小肠。在两名患者中，基于阿苯达唑的治疗使粪便中没有微孢子虫孢子，而对肠道疾病没有影响。大肠杆菌的致病作用。有免疫能力的个体的肠道仍有待证明。 展开▼

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《Journal of Clinical Microbiology》 |1998年第1期|共4页

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Laurent Raynaud; Frederic Delbac; Veronique Broussolle; Meja Rabodonirina; Veronique Girault; Martine Wallon; Gregoire Cozon; Christian P. Vivares; Fran?ois Peyron;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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1. Specific PCR assay for direct detection of intestinal microsporidia Enterocytozoon bieneusi and Encephalitozoon intestinalis in fecal specimens from human immunodeficiency virus-infected patients. [J] . C Ombrouck, L Ciceron, S Biligui, Journal of Clinical Microbiology . 1997,第3期

机译：用于直接检测人免疫缺陷病毒感染患者粪便中肠小孢子虫肠小孢子虫比氏小肠和脑小肠小肠的特异性PCR检测法。

2. Renal Encephalitozoon (Septata) intestinalis infection in a patient with AIDS. Post-mortem identification by means of transmission electron microscopy and PCR. [J] . Boldorini R, Monga G, Tosoni A, Virchows Archiv: an international journal of pathology . 1998,第6期

机译：艾滋病患者的肾性脑病（Septata）小肠感染。通过透射电子显微镜和PCR进行验尸鉴定。

3. Polyclonal and monoclonal antibody and PCR-amplified small-subunit rRNA identification of a microsporidian, Encephalitozoon hellem, isolated from an AIDS patient with disseminated infection. [J] . G S Visvesvara, G J Leitch, A J da Silva, Journal of Clinical Microbiology . 1994,第11期

机译：多克隆和单克隆抗体，以及PCR扩增的小孢子虫小脑亚型rRNA的鉴定，从患有传播感染的艾滋病患者中分离出。

4. Low pressure UV disinfection studies of the microsporidial pathogens Encephalitozoon intestinalis, E. hellem and E. cuniculi: comparison of the in vitro microwell coverslip and in vitro RT-PCR viability assays for determining UV log reduction data [C] . Marilyn M. Marshall, Jackie Moffett, Wayne L. Nicholson, Water quality technology conference;WQTC . 2001

机译：对小孢子病原体肠小脑虫，大肠埃希菌和黄瓜小肠埃希菌的低压紫外线消毒研究：体外微孔盖玻片和体外RT-PCR活力测定的比较，以确定紫外线对数减少数据

5. Infectivity and pathogenesis of Encephalitozoon intestinalis in an in vivo and in vitro porcine model. [D] . Moorkanat, Gopakumar. 2008

机译：在体内和体外猪模型中小肠脑炎的感染性和发病机理。

6. Identification of Encephalitozoon intestinalis in Travelers with Chronic Diarrhea by Specific PCR Amplification [O] . Laurent Raynaud, Frederic Delbac, Veronique Broussolle, 1998

机译：特异性PCR扩增鉴定慢性腹泻旅客脑小肠

7. Identification of Genotypes of Giardia intestinalis Isolates from Dogs in Japan by Direct Sequencing of the PCR Amplified Glutamate Dehydrogenase Gene. [O] . Niichiro ABE, Isao KIMATA, Motohiro ISEKI 2003

机译：通过直接测序PCR扩增的谷氨酸脱氢酶基因的测序鉴定JAIRDIA INTESTILALINIS与日本犬分离物的基因型。

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6. 改进"PCR扩增DNA片段及凝胶电泳鉴定"实验,培养学生科学思维和探究能力 [C] . 张瑾 . 北京市教育学会2018学术年会 . 2018

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3. SPECIFIC MONOCLONAL ANTIBODY 2E5 FOR THE DIAGNOSIS AND CHARACTERIZATION OF HUMAN AND ANIMAL MICROSPORIDIOSIS CAUSED BY ENCEPHALITOZOON INTESTINALIS. [P] . 外国专利： ES2324087B1 . 2010-04-26

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Identification of Encephalitozoon intestinalis in Travelers with Chronic Diarrhea by Specific PCR Amplification

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