Diversity within the VP4 Gene of Rotavirus P[8] Strains: Implications for Reverse Transcription-PCR Genotyping

Miren Iturriza-Gómara; Jon Green; David W. G. Brown; Ulrich Desselberger; James J. Gray

首页> 外文期刊>Journal of Clinical Microbiology >Diversity within the VP4 Gene of Rotavirus P[8] Strains: Implications for Reverse Transcription-PCR Genotyping

【24h】

Diversity within the VP4 Gene of Rotavirus P[8] Strains: Implications for Reverse Transcription-PCR Genotyping

机译：轮状病毒P [8]株VP4基因内的多样性：对逆转录PCR PCR基因分型的影响。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A degenerate version (1T1-D) of the rotavirus P[8]-specific primer (1T-1) allowed strains previously untypeable due to the accumulation of point mutations at the primer binding site to be P typed by reverse transcription-PCR. Sequencing of the cDNA followed by sequence alignment and phylogenetic analysis identified lineages and sublineages within the rotavirus P[8] types, while the use of 1T-1 or 1T-1D primers did not yield viral clusters in any particular lineage.

机译：轮状病毒P [8]-特异性引物（1T-1）的简并版本（1T1-D）允许以前无法分型的菌株，因为在引物结合位点上积累了点突变，因此可以通过逆转录PCR进行P型分型。 cDNA测序，随后进行序列比对和系统进化分析，确定了轮状病毒P [8]类型内的谱系和亚谱系，而使用1T-1或1T-1D引物在任何特定谱系中均未产生病毒簇。

著录项

来源
《Journal of Clinical Microbiology》 |2000年第2期|共4页
作者
Miren Iturriza-Gómara; Jon Green; David W. G. Brown; Ulrich Desselberger; James J. Gray;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类医学微生物学（病原细菌学、病原微生物学）;
关键词

相似文献

外文文献
中文文献
专利

1. Diversity of VP7, VP4, VP6, NSP2, NSP4, and NSP5 genes of porcine rotavirus C: phylogenetic analysis and description of potential new VP7, VP4, VP6, and NSP4 genotypes [J] . Moutelikova Romana, Prodelalova Jana, Dufkova Lucie Archives of virology . 2015,第7期

机译：猪轮状病毒C的VP7，VP4，VP6，NSP2，NSP4和NSP5基因的多样性：系统发育分析和潜在的新VP7，VP4，VP6和NSP4基因型的描述
2. VP4 and VP7 Genotyping by Reverse Transcription-PCR of Human Rotavirus in Mexican Children with Acute Diarrhea [J] . Araceli Rodríguez Castillo, Andrés Velasco Villa, JoséErnesto Ramírez González, Journal of Clinical Microbiology . 2000,第10期

机译：墨西哥儿童急性腹泻中人轮状病毒逆转录-PCR的VP4和VP7基因分型
3. Sequencing and Phylogenetic Analysis of Human Genotype P[6] Rotavirus Strains Detected in Hungary Provides Evidence for Genetic Heterogeneity within the P[6] VP4 Gene [J] . Krisztián Bányai, Vito Martella, Ferenc Jakab, Journal of Clinical Microbiology . 2004,第9期

机译：在匈牙利检测到的人类基因型P [6]轮状病毒菌株的测序和系统发育分析为P [6] VP4基因内的遗传异质性提供了证据
4. Whole-genome sequencing of porcine Rotavirus A, B and C reveals changing genetic diversity and new genotypes [C] . Elizabeth Poulsen, Jianfa Bai, Joe Anderson, Annual^Meeting of the American Association^of^Swine^Veterinarians. . 2014

机译：猪轮状病毒A，B和C的全基因组测序显示出变化的遗传多样性和新基因型
5. Genetic diversity within Bradyrhizobia populations that nodulate soybean genotypes in Africa. [D] . Abaidoo, Robert Clement. 1997

机译：根瘤菌种群中的遗传多样性使非洲大豆基因型结节。
6. Note: Diversity within the VP4 Gene of Rotavirus P8 Strains: Implications for Reverse Transcription-PCR Genotyping [O] . Miren Iturriza-Gómara, Jon Green, David W. G. Brown, 2000

机译：注意：轮状病毒P 8株VP4基因内的多样性：逆转录PCR PCR基因分型的意义
7. VP4 and VP7 Genotyping by Reverse Transcription-PCR of Human Rotavirus in Mexican Children with Acute Diarrhea [O] . Araceli Rodríguez Castillo, Andrés Velasco Villa, JoséErnesto Ramírez González, 2000

机译：VP4和VP7基因分型通过急性腹泻的墨西哥儿童逆转录-PCR逆转录-PCR

Diversity within the VP4 Gene of Rotavirus P[8] Strains: Implications for Reverse Transcription-PCR Genotyping

摘要

著录项

相似文献

相关主题

期刊订阅