Cryopreserved Cell Monolayers for Rapid Detection of Herpes Simplex Virus and Influenza Virus

Yung T. Huang; Huimin Yan; Yan Sun; Joseph A. Jollick Jr.; Heather Baird

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Cryopreserved Cell Monolayers for Rapid Detection of Herpes Simplex Virus and Influenza Virus

机译：冷冻保存的细胞单层，可快速检测单纯疱疹病毒和流感病毒

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Cryopreserved cell monolayers are a new cell culture technology intended to ensure the availability of cells in the laboratory for virus detection. Two cryopreserved cell monolayers, ELVIS for the detection of herpes simplex virus (HSV) and R-Mix for the detection of influenza virus, were evaluated. The results indicated that fresh and cryopreserved cell monolayers are comparable in sensitivity for the detection of HSV and influenza virus. The cells retain the same level of sensitivity for up to 4 months at ?80°C.

机译：冷冻保存的细胞单层是一种新的细胞培养技术，旨在确保实验室中的细胞可用于病毒检测。评估了两个冷冻保存的细胞单层，用于检测单纯疱疹病毒（HSV）的ELVIS和用于检测流感病毒的R-Mix。结果表明，新鲜的和冷冻保存的细胞单层在检测HSV和流感病毒方面具有可比性。电池在？80°C下保持相同水平的灵敏度长达4个月。

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《Journal of Clinical Microbiology》 |2002年第11期|共3页
作者
Yung T. Huang; Huimin Yan; Yan Sun; Joseph A. Jollick Jr.; Heather Baird;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Rapid quantitative PCR assays for the simultaneous detection of herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein-Barr virus, and human herpesvirus 6 DNA in blood and other clinical specimens. [J] . Engelmann I, Petzold DR, Kosinska A, Journal of Medical Virology . 2008,第3期

机译：快速定量PCR分析法可同时检测血液和其他临床标本中的单纯疱疹病毒，水痘带状疱疹病毒，巨细胞病毒，爱泼斯坦-巴尔病毒和人疱疹病毒6 DNA。
2. Detection of herpes simplex virus type 1, herpes simplex virus type 2 and varicella-zoster virus in skin lesions. Comparison of real-time PCR, nested PCR and virus isolation. [J] . Schmutzhard J, Merete Riedel H, Zweygberg Wirgart B, Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology . 2004,第2期

机译：检测皮肤病变中的1型单纯疱疹病毒和2型单纯疱疹病毒和水痘带状疱疹病毒。实时PCR，巢式PCR和病毒分离的比较。
3. Mapping of a herpes simplex virus type 2-encoded function that affects the susceptibility of herpes simplex virus-infected target cells to lysis by herpes simplex virus-specific cytotoxic T lymphocytes. [J] . V C Carter, S R Jennings, P L Rice, Journal of Virology . 1984,第3期

机译：疱疹病毒型2编码功能的映射，影响单纯疱疹病毒感染的靶细胞的易感性通过疱疹病毒特异性细胞毒性T淋巴细胞裂解。
4. Antiviral Activity of Cisplatin Derivatives of Methotrexate Against Vaccinia Virus, Varicella Zoster Virus (VZV), Reovirus ST3, and Herpes Simplex Virus (HSV-1) [C] . Michael R. Roner, Charles E. Carraher Jr., Salima Dhanji PMSE Symposia . 2005

机译：甲氨蝶呤对痘苗病毒，植物疱疹病毒（VZV），reoVirus ST3和单纯疱疹病毒（HSV-1）的抗病毒活性
5. Cellular Substrates of the Herpes Simplex Virus Ser/Thr Kinase Us3: Identification, Characterization and Implications for Virus Biology. [D] . Chuluunbaatar, Uyanga. 2013

机译：单纯疱疹病毒Ser / Thr激酶Us3的细胞底物：病毒生物学的鉴定，表征和意义。
6. Cryopreserved Cell Monolayers for Rapid Detection of Herpes Simplex Virus and Influenza Virus [O] . Yung T. Huang, Huimin Yan, Yan Sun, 2002

机译：冷冻保存的细胞单层可快速检测单纯疱疹病毒和流感病毒
7. Cryopreserved Cell Monolayers for Rapid Detection of Herpes Simplex Virus and Influenza Virus [O] . Huang, Yung T., Yan, Huimin, Sun, Yan, 2002

机译：冷冻保存的细胞单层，可快速检测单纯疱疹病毒和流感病毒

Cryopreserved Cell Monolayers for Rapid Detection of Herpes Simplex Virus and Influenza Virus

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