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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of Two Commercial Assays with Expert Microscopy for Confirmation of Symptomatically Diagnosed Malaria
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Comparison of Two Commercial Assays with Expert Microscopy for Confirmation of Symptomatically Diagnosed Malaria

机译：两种商业化验与专家显微镜对有症状诊断的疟疾确认的比较

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Conventional light microscopy has been the established method for malaria diagnosis. However, recently several nonmicroscopic rapid diagnostic tests have been developed for situations in which reliable microscopy may not be available. This study was conducted to evaluate the diagnostic performance of a recently introduced ICT Malaria Pf/Pv test. This assay detects Plasmodium falciparum histidine-rich protein 2 antigen (PfHRP-2) for P. falciparum diagnosis and pan-malarial antigen for P. vivax diagnosis. In this study we compared the performance of ICT Malaria Pf/Pv with microscopy of Giemsa-stained blood films and with an OptiMAL test that detects Plasmodium lactate dehydrogenase (pLDH) antigen. A total of 750 clinically suspected malaria patients were examined at local health centers in Kuwait. Both the antigen tests had a high degree of specificity (>98%) for detection of malaria infection. However, they were less sensitive than microscopy. Compared with microscopy the ICT Malaria PF/pf test failed to detect malaria infection in 93 (34%) of 271 malaria patients (11% of patients with P. falciparum and 37% of patients with P. vivax) and the OptiMAL test failed to detect malaria infection in 41 (15%) of 271 malaria patients (7% of patients with P. falciparum and 13% of patients with P. vivax). The sensitivities of the ICT Malaria Pf/Pv and OptiMAL tests for detection of P. falciparum infection were 81 and 87%, and those for detecting P. vivax were 58 to 79%, respectively. The sensitivity of the ICT Malaria Pf/Pv and OptiMAL tests decreased significantly to 23 and 44%, respectively, at parasite densities of <500/μl. Both of the tests also produced a number of false-positive results. Overall, the performance of the OptiMAL test was better than that of the ICT Malaria Pf/Pv test. However, our results raise particular concern over the sensitivity of the ICT Malaria Pf/Pv test for detection of P. vivax infection. Further developments appear necessary to improve the performance of the ICT Malaria Pf/Pv test.
机译：常规的光学显微镜已成为疟疾诊断的既定方法。然而，近来已经针对无法使用可靠的显微镜的情况开发了几种非显微镜的快速诊断测试。进行这项研究是为了评估最近推出的ICT疟疾Pf / Pv测试的诊断性能。该检测方法可检测出 P的恶性疟原虫组氨酸富集蛋白2抗原（PfHRP-2）。恶性疟原虫的诊断和 P的泛疟抗原。 vivax 诊断。在这项研究中，我们将ICT疟疾Pf / Pv的性能与Giemsa染色的血膜显微镜进行了比较，并与检测乳杆菌乳酸脱氢酶（pLDH）抗原的OptiMAL测试进行了比较。在科威特当地的卫生中心对总共750名临床怀疑的疟疾患者进行了检查。两种抗原测试均具有检测疟疾感染的高度特异性（> 98％）。但是，它们不如显微镜敏感。与显微镜相比，ICT疟疾PF / pf检测未能检测到271名疟疾患者中的93名（34％）疟疾感染（11％的恶性疟原虫患者和37％的患者）间日疟原虫）和OptiMAL测试未能检测到271名疟疾患者中的41名（15％）疟疾感染（恶性疟原虫患者的7％和恶性疟原虫的13％ em>间日疟原虫）。 ICT疟疾Pf / Pv和OptiMAL检测对 P的敏感性。恶性疟原虫的感染率分别为81％和87％。居间率分别为58％至79％。寄生虫密度小于500 /μl时，ICT疟疾Pf / Pv和OptiMAL测试的灵敏度分别显着降低至23％和44％。两项测试还产生了许多假阳性结果。总体而言，Optimal测试的性能优于ICT疟疾Pf / Pv测试。但是，我们的结果引起了人们对ICT疟疾Pf / Pv测试对检测 P的敏感性的特别关注。间质感染。似乎有必要进一步发展以提高ICT疟疾Pf / Pv测试的性能。

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