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首页> 外文期刊>Journal of Clinical Microbiology >Use of 16S rRNA Gene Sequencing for Rapid Identification and Differentiation of Burkholderia pseudomallei and B. mallei
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Use of 16S rRNA Gene Sequencing for Rapid Identification and Differentiation of Burkholderia pseudomallei and B. mallei

机译：16S rRNA基因测序的应用快速鉴定和鉴别伯克霍尔德菌假芽孢杆菌和B. Mallei

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Burkholderia pseudomallei and B. mallei, the causative agents of melioidosis and glanders, respectively, are designated category B biothreat agents. Current methods for identifying these organisms rely on their phenotypic characteristics and an extensive set of biochemical reactions. We evaluated the use of 16S rRNA gene sequencing to rapidly identify these two species and differentiate them from each other as well as from closely related species and genera such as Pandoraea spp., Ralstonia spp., Burkholderia gladioli, Burkholderia cepacia, Burkholderia thailandensis, and Pseudomonas aeruginosa. We sequenced the 1.5-kb 16S rRNA gene of 56 B. pseudomallei and 23 B. mallei isolates selected to represent a wide range of temporal, geographic, and origin diversity. Among all 79 isolates, a total of 11 16S types were found based on eight positions of difference. Nine 16S types were identified in B. pseudomallei isolates based on six positions of difference, with differences ranging from 0.5 to 1.5 bp. Twenty-two of 23 B. mallei isolates showed 16S rRNA gene sequence identity and were designated 16S type 10, whereas the remaining isolate was designated type 11. This report provides a basis for rapidly identifying and differentiating B. pseudomallei and B. mallei by molecular methods.
机译：假伯克霍尔德氏菌和 B。类鼻oid病和腺体的致病因子Mallei 被指定为B类生物威胁因子。鉴定这些生物的当前方法依赖于它们的表型特征和广泛的生化反应。我们评估了使用16S rRNA基因测序来快速鉴定这两个物种并将它们彼此以及与紧密相关的物种和属（例如 Pandoraea spp。， Ralstonia spp。，剑兰伯克霍尔德氏菌，洋葱伯克霍尔德氏菌，伯克霍尔德氏菌泰国种和铜绿假单胞菌。我们对56 B的1.5 kb 16S rRNA基因进行了测序。 pseudomallei 和23 B。被选为代表广泛的时间，地理和起源多样性的Mallei 菌株。在所有79个分离株中，基于八个差异位置共发现11种16S类型。在 B中识别出9种16S类型。假小麦芽孢杆菌是根据六个差异位点分离的，差异在0.5到1.5 bp之间。 B 23个中的22个。 Mallei 分离株表现出16S rRNA基因序列同一性，被命名为16S类型10，而其余分离株被命名为11型。该报告为快速鉴定和区分 B提供了基础。假苹果花和 B。分子生物学方法。

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