Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR

Petra Wolffs; Halfdan Grage; Oskar Hagberg; Peter R?dstr?m

首页> 外文期刊>Journal of Clinical Microbiology >Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR

【24h】

Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR

机译：DNA聚合酶及其缓冲液系统对实时定量PCR的影响

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.

机译：对五个DNA聚合酶-缓冲液系统对实时PCR的影响进行的研究表明，DNA聚合酶和缓冲液系统的选择都会影响扩增效率以及检测窗口。对于不同系统，数据的分析可重复性发生了明显变化，使我们得出结论，基于单一数据集标准曲线进行定量测量可能会导致重大错误。

著录项

来源
《Journal of Clinical Microbiology》 |2004年第1期|共4页
作者
Petra Wolffs; Halfdan Grage; Oskar Hagberg; Peter R?dstr?m;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类医学微生物学（病原细菌学、病原微生物学）;
关键词

相似文献

外文文献
中文文献
专利

1. Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR [J] . Petra Wolffs, Halfdan Grage, Oskar Hagberg, Journal of Clinical Microbiology . 2004,第1期

机译：DNA聚合酶及其缓冲液系统对实时定量PCR的影响
2. Proposal for a performance factor to characterize real-time PCR systems, evaluate premixed DNA-polymerases and assign minimal performance criteria for individual quantitative PCR runs. [J] . Koeppel R, Zimmerli F. European food research and technology =: Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A . 2010,第5期

机译：建议使用性能因子来表征实时PCR系统，评估预混合的DNA聚合酶并为单个定量PCR运行指定最低性能标准。
3. Proposal for a performance factor to characterize real-time PCR systems, evaluate premixed DNA-polymerases and assign minimal performance criteria for individual quantitative PCR runs [J] . René Köppel, Franziska Zimmerli European Food Research and Technology . 2010,第5期

机译：建议使用性能因子来表征实时PCR系统，评估预混的DNA聚合酶并为单个定量PCR运行指定最低性能标准
4. Picoliter Real-Time Quantitative Polymerase Chain Reaction (qPCR) in an All-Fiber System [C] . Xuegang Li, Linh V. Nguyen, Kelly Hill, Asia Communications and Photonics Conference . 2019

机译：全光纤系统中的Picoliter实时定量聚合酶链反应（qPCR）
5. Rapid and sensitive detection of aflatoxin in animal feeds and food grains using immunomagnetic bead based recovery and real-time immuno quantitative PCR (RT-iqPCR) assay. [D] . Babu, Dinesh. 2010

机译：使用基于免疫磁珠的回收率和实时免疫定量PCR（RT-iqPCR）分析快速，灵敏地检测动物饲料和粮食中的黄曲霉毒素。
6. Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR [O] . Petra Wolffs, Halfdan Grage, Oskar Hagberg, 2004

机译：DNA聚合酶及其缓冲液系统对实时定量PCR的影响
7. Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR [O] . 2015

机译：DNa聚合酶及其缓冲系统对定量实时pCR的影响
8. Evaluation of Real-Time Quantitative Polymerase Chain Reaction (qPCR) to Determine Escherichia coli Concentrations at Two Lake Erie Beaches [R] . Kephart, C. M., Bushon, R. N. 2009

机译：评估实时定量聚合酶链反应（qpCR）以确定两个伊利湖海滩的大肠杆菌浓度

Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR

摘要

著录项

相似文献

相关主题

期刊订阅