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首页> 外文期刊>Journal of Clinical Microbiology >Typing of group A streptococci by random amplified polymorphic DNA analysis.
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Typing of group A streptococci by random amplified polymorphic DNA analysis.

机译：通过随机扩增多态性DNA分析对A组链球菌进行分型。

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Random amplified polymorphic DNA (RAPD) analysis was evaluated in comparison with restriction endonuclease analysis (REA) of genomic DNA and serotyping in the typing of 160 epidemiologically unrelated group A streptococci (GAS). Amplification of genomic DNA of GAS was performed with a single primer with an arbitrarily selected nucleotide sequence of 12 nucleotides. In total, 31 RAPD patterns and 15 REA patterns were observed among the isolates studied. The results of RAPD analysis were in accordance with the results of REA for 86% of the isolates, as both methods identified 15 different strains among 138 isolates. However, RAPD analysis differentiated 16 additional strains among 22 isolates. RAPD analysis was somewhat better than REA for differentiation of isolates of the same and different serotypes. However, not all of the serotypes were differentiated by RAPD analysis either. In conclusion, RAPD analysis provides a practical alternative for genomic typing of GAS. It can be recommended for the typing of GAS, especially if used in parallel with serotyping.
机译：与基因组DNA的限制性内切核酸酶分析（REA）和160种流行病学无关的A组链球菌（GAS）的分型中的血清分型相比，对随机扩增多态性DNA（RAPD）分析进行了评估。 GAS基因组DNA的扩增是使用具有12个核苷酸的任意选择核苷酸序列的单个引物进行的。在所研究的菌株中，共观察到31种RAPD模式和15种REA模式。 RAPD分析的结果与86％分离株的REA结果一致，因为两种方法均在138个分离株中鉴定出15种不同的菌株。但是，RAPD分析在22个分离株中区分了16个其他菌株。对于相同和不同血清型的分离株，RAPD分析比REA更好。但是，也没有通过RAPD分析区分所有血清型。总之，RAPD分析为GAS的基因组分型提供了一种实用的选择。推荐将其用于GAS的分类，尤其是与血清分型同时使用时。

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