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首页> 外文期刊>Journal of Clinical Microbiology >Characteristics of Escherichia coli strains belonging to enteropathogenic E. coli serogroups isolated in Italy from children with diarrhea.
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Characteristics of Escherichia coli strains belonging to enteropathogenic E. coli serogroups isolated in Italy from children with diarrhea.

机译：在意大利从腹泻儿童中分离出的属于肠致病性大肠杆菌血清群的大肠杆菌菌株的特征。

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Fifty-five Escherichia coli strains belonging to enteropathogenic E. coli (EPEC) serogroups were examined for phenotypic and genetic factors associated with virulence. The strains were isolated in Italy from children with diarrhea and identified as EPEC by clinical laboratories using commercially available antisera. O:H serotyping showed that 35 strains (27 of O26, O111, and O128 serogroups) belonged to 11 serotypes considered to be classical EPEC O:H serotypes. The other 20 isolates were classified as 15 nonclassical EPEC O:H serotypes. All the potential EPEC virulence factors associated with bacterial adhesion (localized adherence, fluorescentactin staining test positivity, presence of the attaching and effacing [eaeA] gene), the production of verotoxin, and the positivity with the enterohemorrhagic E. coli probe were significantly more frequent among isolates belonging to classical than nonclassical serotypes. Strains displaying an aggregative adhesion and hybridizing with the enteroaggregative DNA probe were found in serogroups O86, O111, and O126. Verotoxin-producing isolates belonged to serogroups O26, O111, and O128. Only one of the isolates hybridized with the EPEC adherence factor (EAF) probe, but 33 strains gave positive results with the eae probe, confirming that the former is more suitable in epidemiological studies in European countries. These results indicate that up to 75% of strains identified as EPEC by commercial antisera may possess potential virulence properties and/or belong to classical EPEC O:H serotypes and suggest that O grouping is still a useful diagnostic tool for presumptive identification of diarrheagenic E. coli in clinical laboratories.
机译：检查属于肠致病性大肠杆菌（EPEC）血清群的55株大肠杆菌的表型和遗传因素与毒力相关。该菌株是在意大利从腹泻儿童中分离出来的，并通过临床实验室使用市售抗血清鉴定为EPEC。 O：H血清分型显示35个菌株（O26，O111和O128血清群中的27个）属于被认为是经典EPEC O：H血清型的11种血清型。其他20种分离株被分类为15种非经典EPEC O：H血清型。与细菌粘附相关的所有潜在的EPEC毒力因子（局部粘附，荧光肌动蛋白染色试验阳性，附着和脱落[eaeA]基因的存在），verotoxin的产生以及肠出血性大肠杆菌探针的阳性率明显更高在属于经典比非经典血清型的分离物中。在血清群O86，O111和O126中发现了具有聚集黏附性并与肠聚集性DNA探针杂交的菌株。产生维毒素的分离株属于血清群O26，O111和O128。仅一种分离株与EPEC粘附因子（EAF）探针杂交，但33株菌株用eae探针给出了阳性结果，证实了前者更适合欧洲国家的流行病学研究。这些结果表明，多达75％的被商业抗血清鉴定为EPEC的菌株可能具有潜在的毒力特性和/或属于经典的EPEC O：H血清型，并且表明O分组仍然是推定鉴定腹泻性E的有用诊断工具。临床实验室中的大肠杆菌。

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