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首页> 外文期刊>Journal of Clinical Microbiology >Differentiation of Mycobacterium ulcerans, M. marinum, and M. haemophilum: Mapping of Their Relationships to M. tuberculosis by Fatty Acid Profile Analysis, DNA-DNA Hybridization, and 16S rRNA Gene Sequence Analysis
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Differentiation of Mycobacterium ulcerans, M. marinum, and M. haemophilum: Mapping of Their Relationships to M. tuberculosis by Fatty Acid Profile Analysis, DNA-DNA Hybridization, and 16S rRNA Gene Sequence Analysis

机译：溃疡分枝杆菌，海藻分枝杆菌和嗜血分枝杆菌的分化：通过脂肪酸谱分析，DNA-DNA杂交和16S rRNA基因序列分析其与结核分枝杆菌的关系图

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摘要

Although Mycobacterium ulcerans, M. marinum, and M. haemophilum are closely related, their exact taxonomic placements have not been determined. We performed gas chromatography of fatty acids and alcohols, as well as DNA-DNA hybridization and 16S rRNA gene sequence analysis, to clarify their relationships to each other and to M. tuberculosis. M. ulcerans and M. marinum were most closely related to one another, and each displayed very strong genetic affinities toM. tuberculosis; they are actually the two mycobacterial species outside the M. tuberculosis complex most closely related to M. tuberculosis. M. haemophilum was more distinct from M. ulcerans and M. marinum, and it appeared to be as related to these two species as to M. tuberculosis. These results are important with regard to the development of diagnostic and epidemiological tools such as species-specific DNA probes and PCR assays for M. ulcerans,M. marinum, and M. haemophilum. In addition, the finding that M. ulcerans and M. marinum are more closely related to M. tuberculosis than are other pathogenic mycobacterial species suggests that they may be evaluated as useful models for studying the pathogenesis of M. tuberculosis. M. marinum may be particularly useful in this regard since strains of this species grow much more rapidly than M. tuberculosis and yet can cause systemic disease in immunocompromised hosts.
机译：虽然是溃疡分枝杆菌， marinum 和 M。嗜血杆菌密切相关，其确切的生物分类位置尚未确定。我们进行了脂肪酸和醇的气相色谱分析，以及DNA-DNA杂交和16S rRNA基因序列分析，以阐明它们之间的相互关系以及与 M的关系。结核。溃疡分枝杆菌和 M。 marin 之间的亲缘关系最密切，并且每个displayed对M的遗传亲和力都很高。结核病;它们实际上是 M之外的两个分枝杆菌物种。结核病复合物与 M最密切相关。结核病。 M。嗜血杆菌与 M的区别更大。溃疡和 M。 marinum ，似乎与这两个物种与 M有关。结核病。这些结果对于开发诊断和流行病学工具（例如针对M的物种特异性DNA探针和PCR分析）非常重要。溃疡， M。 marinum 和 M。嗜血杆菌。另外，发现 M。溃疡和 M。 marinum 与 M密切相关。结核比其他致病性分枝杆菌物种更表明，它们可能被评估为研究 M发病机理的有用模型。结核病。 M。 marinum 在这方面可能特别有用，因为该菌种的生长比 M快得多。结核，但仍可能在免疫功能低下的宿主中引起全身性疾病。

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