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首页> 外文期刊>Journal of Clinical Microbiology >Identification of Virulence Genes Linked with Diarrhea Due to Atypical Enteropathogenic Escherichia coli by DNA Microarray Analysis and PCR
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Identification of Virulence Genes Linked with Diarrhea Due to Atypical Enteropathogenic Escherichia coli by DNA Microarray Analysis and PCR

机译：DNA微阵列分析和PCR鉴定与非典型肠道致病性大肠杆菌引起的腹泻相关的致病基因

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The role of atypical enteropathogenic Escherichia coli (EPEC) in childhood diarrhea is controversial. The aim of the present study was to search for genes linked with diarrhea in atypical EPEC strains from a case-control study among Norwegian children. Using DNA microarray analysis, genomic DNAs from strains isolated from children with (n = 37) and without (n = 20) diarrhea were hybridized against 242 different oligonucleotide probes specific for 182 virulence genes or markers from all known E. coli pathotypes. PCR was performed to test the strains for seven putative virulence genes not included in the microarray panel. The OI-122 gene efa1/lifA was the gene with the strongest statistical association with diarrhea (P = 0.0008). Other OI-122 genes (set/ent, nleB, and nleE) and genes with other locations (lpfA, paa, ehxA, and ureD) were also associated with diarrheal disease. The phylogenetic marker gene yjaA was negatively associated with diarrhea (P = 0.0004). Atypical EPEC strains could be classified in two main virulence groups based on their content of OI-122, lpfA, and yjaA genes. Among children with diarrhea, atypical EPEC isolates belonging to virulence group I (OI-122 and lpfA positive, yjaA negative) were the most common, while the majority of isolates from healthy children were classified as virulence group II strains (OI-122 negative, lpfA and yjaA positive; P < 0.001). In conclusion, using DNA microarray analysis to determine the virulence gene profile of atypical EPEC isolates, several genes were found to be significantly associated with diarrhea. Based on their composition of virulence genes, the majority of strains could be classified in two virulence groups, of which one was seen mainly in children with diarrhea.
机译：非典型肠致病性大肠埃希菌在儿童腹泻中的作用是有争议的。本研究的目的是通过一项在挪威儿童中进行的病例对照研究，寻找与非典型EPEC菌株腹泻有关的基因。使用DNA芯片分析，将分离自患有（ n = 37）和没有（ n = 20）腹泻儿童的菌株的基因组DNA与针对182的242种不同寡核苷酸探针杂交来自所有已知 E。的毒力基因或标记。大肠型进行PCR以测试菌株中七个未包括在微阵列面板中的毒力基因。 OI-122基因 efa1 / lifA 是与腹泻相关性最强的基因（ P = 0.0008）。其他OI-122基因（ set / ent ， nleB 和 nleE ）和其他位置的基因（ lpfA ， paa ， ehxA 和 ureD ）也与腹泻病相关。系统发育标记基因 yjaA 与腹泻呈负相关（ P = 0.0004）。根据OI-122， lpfA 和 yjaA 基因的含量，可以将非典型EPEC菌株分为两个主要毒力组。在腹泻儿童中，最常见的是属于I型毒力的非典型EPEC分离株（OI-122和 lpfA 阳性， yjaA 阴性），而大多数健康分离株将儿童分类为II型毒力毒株（OI-122阴性， lpfA 和 yjaA 阳性; P <0.001）。总之，使用DNA芯片分析来确定非典型EPEC分离株的毒力基因谱，发现有几个基因与腹泻显着相关。根据其毒力基因的组成，大多数菌株可分为两个毒力组，其中一个毒株主要出现在腹泻儿童中。

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