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首页> 外文期刊>Journal of Clinical Microbiology >Testing the Interaction between NOD-2 Status and Serological Response to Mycobacterium paratuberculosis in Cases of Inflammatory Bowel Disease
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Testing the Interaction between NOD-2 Status and Serological Response to Mycobacterium paratuberculosis in Cases of Inflammatory Bowel Disease

机译：测试炎症性肠病病例中NOD-2状态与副结核分枝杆菌血清学反应之间的相互作用

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In a population-based case-control study we have previously shown that 14% of healthy Manitobans carry one or two mutations in the NOD-2 locus, a gene highly associated with Crohn's disease (CD). The NOD-2 protein is the receptor responsible for recognition of bacterial peptidoglycans, and it is plausible that NOD-2 is involved in the recognition of mycobacteria. Thirty-seven percent of Manitobans with CD had ≥1 NOD-2 mutation, leading to a threefold increased risk of CD for single-mutant carriers and a 30-fold increased risk for double-mutant carriers. In the same population groups, we assessed the seroprevalence for Mycobacterium paratuberculosis and found it to be 35%, with no differences between CD, ulcerative colitis (UC), and controls. Because of high rates of CD and UC in Manitoba, we assessed whether there was an interaction between carrying a NOD-2 mutation and M. paratuberculosis seropositivity. An enzyme-linked immunosorbent assay for serum antibodies to M. paratuberculosis in cattle was adapted for human use. DNA was purified from whole blood. Subjects were genotyped for three NOD-2 variants, G908R, Cins1007fs, and R702W. Multivariate logistic regression analysis showed that NOD-2 gene mutations significantly associated with CD, but M. paratuberculosis serology did not. Furthermore, there was no interaction between NOD-2 mutation status and M. paratuberculosis serology status. For those with the NOD-2 mutation, the likelihood of CD subjects having positive M. paratuberculosis serology was similar to that of controls (odds ratio, 1.31; 95% confidence interval, 0.55-3.11). No interaction could be proven for UC or by combining CD and UC compared to controls. In conclusion, we could not find an interaction between the NOD-2 genotype and M. paratuberculosis serology in relationship to CD or UC.
机译：在一项基于人群的病例对照研究中，我们先前已经显示14％的健康马尼托班在NOD-2基因座中携带一个或两个突变，该基因与克罗恩病（CD）高度相关。 NOD-2蛋白是负责识别细菌肽聚糖的受体，似乎可以认为NOD-2参与了分枝杆菌的识别。患有CD的马尼托班中有37％的NOD-2突变≥1，导致单突变携带者CD患病风险增加三倍，双突变携带者CD患病风险增加30倍。在同一人群中，我们评估了副结核分枝杆菌的血清阳性率，发现其为35％，CD，溃疡性结肠炎（UC）和对照组之间无差异。由于曼尼托巴省CD和UC的发生率很高，我们评估了携带NOD-2突变与 M之间是否存在相互作用。副结核病血清阳性。酶联免疫吸附法检测 M的血清抗体。牛的副结核病适合人类使用。从全血中纯化DNA。对受试者的三种NOD-2变体G908R，Cins1007fs和R702W进行基因分型。多变量logistic回归分析表明，NOD-2基因突变与CD显着相关，但与 M相关。副结核病没有。此外，NOD-2突变状态与 M之间没有相互作用。副结核病的血清学状况对于那些具有NOD-2突变的人，CD受试者M阳性的可能性。副结核病血清学与对照组相似（优势比为1.31; 95％置信区间为0.55-3.11）。与对照组相比，UC或与CD和UC结合使用无法证明相互作用。总之，我们找不到NOD-2基因型与 M之间的相互作用。 CD或UC相关的副结核病血清学。

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