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首页> 外文期刊>Journal of Clinical Microbiology >Genetic Rearrangements of Tn1546-Like Elements in Vancomycin-Resistant Enterococcus faecium Isolates Collected from Hospitalized Patients over a Seven-Year Period
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Genetic Rearrangements of Tn1546-Like Elements in Vancomycin-Resistant Enterococcus faecium Isolates Collected from Hospitalized Patients over a Seven-Year Period

机译：七年期间从住院患者收集的耐万古霉素肠球菌粪便中Tn1546-like元素的遗传重排。

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The heterogeneity of Tn1546 results from point mutations, deletions, and the integration of insertion sequence (IS) elements. Among these variations, the presence of IS elements accounts for much of the heterogeneity. Such a rearrangement could play a key role in the evolution of the vanA gene cluster, and hence, it may modify its transferability. In this study, we characterized the consequence of Tn1546 in vanA-containing Enterococcus faecium isolates collected from patients over time. From 1998 to 2004, 57 vanA-containing E. faecium isolates were collected from hospitalized patients at Ajou University Hospital in Korea. PCR amplification of internal regions of Tn1546 was performed, and both DNA strands were directly sequenced by the dideoxy termination method. All isolates were divided into three main types, including the prototype, according to the distribution of IS elements integrated into Tn1546 elements. Type I was characterized by an IS1542 insertion in the orf2-vanR intergenic region and an IS1216V insertion in the vanX-vanY intergenic region. Type II was represented by the presence of two copies of IS1216V at the 3′ end of IS1542 and in the vanX-vanY intergenic region, as well as IS1542 in the orf2-vanR intergenic region. Seventeen strains isolated from 1998 to 2000 represented type I, and 38 strains isolated from 2000 to 2004 represented type II. The remaining two isolates were the prototype. The tendency for the rearrangement of Tn1546 was that the sequences were shortened as time passed, especially at the left or the right end, and hence, this could gradually modulate their transferability.
机译：Tn 1546 的异质性是由点突变，缺失和插入序列（IS）元件的整合导致的。在这些变体中，IS元素的存在是造成异质性的主要原因。这种重排可能在 vanA 基因簇的进化中起关键作用，因此可能会改变其可转移性。在这项研究中，我们表征了随时间从患者体内收集的，含有 vanA 的粪肠球菌分离株中Tn 1546 的结果。从1998年到2004年，有57个包含 vanA 的 E。从韩国阿久大学医院住院患者的粪便中分离出粪便。对Tn 1546 的内部区域进行PCR扩增，并通过双脱氧终止法直接对两条DNA链进行测序。根据整合到Tn 1546 元素中的IS元素的分布，将所有分离物分为原型等三种主要类型。 I型的特征是在 orf2-vanR 基因间区域插入了IS 1542 ，在 vanX-vanY中插入了IS 1216V 基因间区域。通过在IS 1542 的3'端和 vanX-vanY 基因间区域存在两个IS 1216V 副本来代表II型，以及 orf2-vanR 基因间区域中的IS 1542 。从1998年至2000年分离的17个菌株代表I型，从2000年至2004年分离的38个菌株代表II型。其余两个分离株是原型。 Tn 1546 的重排趋势是随着时间的流逝，序列会缩短，尤其是在左端或右端，因此可以逐渐调节其可转移性。

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