Generic Detection of Coronaviruses and Differentiation at the Prototype Strain Level by Reverse Transcription-PCR and Nonfluorescent Low-Density Microarray

Luciano Kleber de Souza Luna; Volker Heiser; Nicolas Regamey; Marcus Panning; Jan Felix Drexler; Sabue Mulangu; Leo Poon; Sigrid Baumgarte; Bert Jan Haijema; Laurent Kaiser; Christian Drosten

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Generic Detection of Coronaviruses and Differentiation at the Prototype Strain Level by Reverse Transcription-PCR and Nonfluorescent Low-Density Microarray

机译：冠状病毒的常规检测和原型菌株水平的反转录PCR和无荧光低密度微阵列的分化。

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A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.

机译：设计了一种无荧光的低成本，低密度寡核苷酸阵列，用于在逆转录（RT）-PCR后检测整个冠状病毒属。检测限为15.7拷贝/反应。严重急性呼吸系统综合症患者的临床检出限为100份/样本。在39名患冠状病毒229E，NL63，OC43或HKU1的儿童中，敏感性与个别实时RT-PCR相等。

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《Journal of Clinical Microbiology》 |2007年第3期|共4页
作者
Luciano Kleber de Souza Luna; Volker Heiser; Nicolas Regamey; Marcus Panning; Jan Felix Drexler; Sabue Mulangu; Leo Poon; Sigrid Baumgarte; Bert Jan Haijema; Laurent Kaiser; Christian Drosten;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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2. Combining Multiplex Reverse Transcription-PCR and a Diagnostic Microarray To Detect and Differentiate Enterovirus 71 and Coxsackievirus A16 [J] . Tsan-Chi Chen, Guang-Wu Chen, Chao Agnes Hsiung, Journal of Clinical Microbiology . 2006,第6期

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4. Serovar and Strain Level Bacterial Differentiation Capabilities for 36 Closely Related Outbreak Strains by Intact Protein LCMS [C] . Melinda A. McFarland, Denis Andrzejewski, Peter Evans, ASMS Conference on Mass Spectrometry and Allied Topics . 2014

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5. Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter. [D] . Zhang, Hai. 2006

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6. Generic Detection of Coronaviruses and Differentiation at the Prototype Strain Level by Reverse Transcription-PCR and Nonfluorescent Low-Density Microarray [O] . Luciano Kleber de Souza Luna, Volker Heiser, Nicolas Regamey, 2007

机译：冠状病毒的常规检测和原型菌株水平的反转录PCR和无荧光低密度微阵列的分化。
7. Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray [O] . de Souza Luna, L. K., Heiser, Volker, Regamey, Nicolas, 2007

机译：逆转录PCR和无荧光低密度微阵列检测冠状病毒并在原型菌株水平上进行分化
8. Detection and differentiation of contamination through a comparison of observed levels in historical environmental sampling data [R] . Denham, D. H., Price, K. R. 1992

机译：通过比较历史环境采样数据中的观察水平来检测和区分污染

Generic Detection of Coronaviruses and Differentiation at the Prototype Strain Level by Reverse Transcription-PCR and Nonfluorescent Low-Density Microarray

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