...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of a New Test, GenoType HelicoDR, for Molecular Detection of Antibiotic Resistance in Helicobacter pylori
【24h】

Evaluation of a New Test, GenoType HelicoDR, for Molecular Detection of Antibiotic Resistance in Helicobacter pylori

机译：评价一种新的基因型HelicoDR测试，用于分子检测幽门螺杆菌中的抗生素耐药性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The eradication rate of Helicobacter pylori by standard therapy is decreasing due to antibiotic resistance, mainly to clarithromycin. Our aim was to provide a new molecular test to guide the treatment of new and relapsed cases. We first studied 126 H. pylori strains for phenotypic (MIC) and genotypic resistance to clarithromycin (rrl mutation) and levofloxacin (gyrA mutation) and then developed a DNA strip genotyping test on the basis of the correlation results and literature data. Clinical strains (n = 92) and gastric biopsy specimens containing H. pylori (n = 105) were tested blindly with the new molecular test GenoType HelicoDR. The presence of mutations or the absence of hybridization with wild-type sequences was predictive, in rrl for clarithromycin resistance in 91 cases (mostly the A2147G mutation) and in gyrA for levofloxacin resistance in 58 cases (mutations at codon 87 or 91). Genotyping revealed a mix of genotypes in 33% of the cases, reflecting a coinfection or selection for resistant mutants. The sensitivity and specificity of detecting resistance were 94% and 99% for clarithromycin and 87% and 98.5% for levofloxacin, respectively. The concordance scores were 0.96 for clarithromycin and 0.94 for levofloxacin. With global resistance rates of 46% for clarithromycin and 25% for levofloxacin, which were observed for consecutive positive biopsy specimens from 2007 and 2008, the positive and negative predictive values for detecting resistance were 99% and 94% for clarithromycin and 96% and 96% for fluoroquinolone. GenoType HelicoDR is efficient at detecting mutations predictive of antibiotic resistance in H. pylori when applied to strains or directly to gastric biopsy specimens.
机译：由于抗生素耐药性（主要是克拉霉素），标准疗法根除幽门螺杆菌的比率正在降低。我们的目的是提供一种新的分子检测方法，以指导新的和复发病例的治疗。我们首先研究了126H。幽门螺杆菌菌株对克拉霉素（ rrl 突变）和左氧氟沙星（ gyrA 突变）的表型（MIC）和基因型抗性，然后在其上进行了DNA条带基因分型测试相关结果和文献数据的基础。临床菌株（ n = 92）和含有 H的胃活检标本。幽门螺杆菌（ n = 105）用新的分子测试GenoType HelicoDR进行盲测。在 rrl 中对克拉霉素耐药的91例患者（主要是A2147G突变）和在 gyrA 中对左氧氟沙星的耐药性是存在突变或与野生型序列不杂交的原因抗性58例（第87或91位密码子突变）。基因分型显示33％的病例中存在基因型混合，反映出合并感染或选择抗性突变体。克拉霉素的抗药性的敏感性和特异性分别为94％和99％，左氧氟沙星为87％和98.5％。克拉霉素的一致性得分为0.96，左氧氟沙星的得分为0.94。从2007年至2008年连续进行的活检样本中，克拉霉素的全球耐药率分别为46％和左氧氟沙星25％，检测耐药的阳性和阴性预测值分别为99％和94％，96％和96氟喹诺酮的％。 GenoType HelicoDR可有效检测可预测 H抗生素耐药性的突变。幽门螺杆菌应用于菌株或直接用于胃活检标本。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号