Broad-Range 16S rRNA PCR with Cerebrospinal Fluid May Be Unreliable for Management of Postoperative Aseptic Meningitis

Virginie Zarrouk; Véronique Leflon-Guibout; Sébastien Robineaux; Michel Kalamarides; Marie-Hélène Nicolas-Chanoine; Olivier Sterkers

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Broad-Range 16S rRNA PCR with Cerebrospinal Fluid May Be Unreliable for Management of Postoperative Aseptic Meningitis

机译：脑脊液的大范围16S rRNA PCR可能对于术后无菌性脑膜炎的治疗不可靠

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We previously demonstrated that discontinuing presumptive antibiotic treatment in cases of negative conventional cultures is safe and effective for patients with postoperative aseptic meningitis (PAM). Here, we prospectively investigated 32 patients with postoperative meningitis. All 26 patients with PAM diagnosed on the basis of conventional cultures demonstrated negative 16S rRNA PCR results. Our results suggest that the PCR technique does not change PAM management.

机译：我们先前证明，在常规培养阴性的情况下停止推定抗生素治疗对术后无菌性脑膜炎（PAM）患者是安全有效的。在这里，我们前瞻性地调查了32例术后脑膜炎患者。根据常规培养诊断出的所有26例PAM患者均显示16S rRNA PCR阴性。我们的结果表明，PCR技术不会改变PAM管理。

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《Journal of Clinical Microbiology》 |2010年第9期|共3页
作者
Virginie Zarrouk; Véronique Leflon-Guibout; Sébastien Robineaux; Michel Kalamarides; Marie-Hélène Nicolas-Chanoine; Olivier Sterkers;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Employment of broad-range 16S rRNA PCR to detect aetiological agents of infection from clinical specimens in patients with acute meningitis - rapid separation of 16S rRNA PCR amplicons without the need for cloning [J] . J. Xu, B. C. Millar, J. E. Moore, Journal of applied microbiology . 2003,第2期

机译：使用大范围16S rRNA PCR检测急性脑膜炎患者临床标本中的感染病原体-无需克隆即可快速分离16S rRNA PCR扩增子
2. Employment of broad-range 16S rRNA PCR to detect aetiological agents of infection from clinical specimens in patients with acute meningitis - rapid separation of 16S rRNA PCR amplicons without the need for cloning [J] . J. Xu, B. C. Millar, J. E. Moore, Journal of applied microbiology . 2003,第2期

机译：使用大范围16S rRNA PCR检测急性脑膜炎患者临床标本中的感染病原体-无需克隆即可快速分离16S rRNA PCR扩增子
3. Meningitis caused by Porphyromonas endodontalis detected by PCR amplification and sequencing of 16S rRNA genes direct from cerebrospinal fluid and cerebral tissue [J] . David W. New, Todd M. Pryce JMM Case Reports . 2015,第5期

机译：通过PCR扩增和测序直接从脑脊液和脑组织的16S rRNA基因检测到由牙本质卟啉菌引起的脑膜炎。
4. Identification of a Acidthiobacillus Species Separated from the Mixed Microbial Populations in Zijinshan Copper Mine Bio-heap Leaching Plant by the Methods of PCR-16S rRNA Gene Sequence Analysis [C] . XIAO Xiang, MA Zhong International Mineral Processing Congress . 2008

机译：PCR-16S rRNA基因序列分析方法从紫金山铜矿生物堆浸厂混合微生物种群中分离到酸性硫杆菌种
5. Soil community analysis along a salt gradient using denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA genes. [D] . Dutta, Partha. 2006

机译：使用PCR扩增的16S rRNA基因的变性梯度凝胶电泳，沿着盐梯度进行土壤群落分析。
6. Broad-Range 16S rRNA PCR with Cerebrospinal Fluid May Be Unreliable for Management of Postoperative Aseptic Meningitis [O] . Virginie Zarrouk, Véronique Leflon-Guibout, Sébastien Robineaux, 2010

机译：脑脊液的大范围16S rRNA PCR可能对于术后无菌性脑膜炎的治疗不可靠
7. Broad-Range 16S rRNA PCR with Cerebrospinal Fluid May Be Unreliable for Management of Postoperative Aseptic Meningitis ▿ [O] . Zarrouk, Virginie, Leflon-Guibout, Véronique, Robineaux, Sébastien, 2010

机译：脑脊液的大范围16S rRNA PCR可能对于术后无菌性脑膜炎的治疗不可靠

Broad-Range 16S rRNA PCR with Cerebrospinal Fluid May Be Unreliable for Management of Postoperative Aseptic Meningitis

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