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首页> 外文期刊>Journal of Clinical Microbiology >Distribution of Non-Locus of Enterocyte Effacement Pathogenic Island-Related Genes in Escherichia coli carrying eae from Patients with Diarrhea and Healthy Individuals in Japan
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Distribution of Non-Locus of Enterocyte Effacement Pathogenic Island-Related Genes in Escherichia coli carrying eae from Patients with Diarrhea and Healthy Individuals in Japan

机译：日本腹泻患者和健康个体携带大肠杆菌的肠上皮细胞感染致病岛相关基因的非基因座分布

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The relationship to diarrhea of genes located on the pathogenicity islands (PAI) other than the locus of enterocyte effacement (LEE) was investigated. Enteropathogenic Escherichia coli (EPEC), the retention of espC on the EspC PAI, the OI-122 genes (efa1/lifA, nleB), the phylogenetic marker gene yjaA, and the bundle-forming pilus gene bfpA on the EPEC adherence factor (EAF) plasmid were studied. E. coli strains carrying the intimin gene (eae) without the Shiga toxin gene, isolated from patients with diarrhea (n = 83) and healthy individuals (n = 38) in Japan, were evaluated using PCR. The genotypes of eae and espC were identified by heteroduplex mobility assay (HMA). The proportions of strains isolated from individuals with and without diarrhea that carried these genes were as follows: bfpA, 13.3 and 7.9%, respectively; espC, 25.3 and 36.8%; efa1/lifA, 32.5 and 13.2%; nleB, 63.9 and 60.5%; yjaA, 42.2 and 55.3%. Statistical significance (P < 0.05) was achieved only for efa1/lifA. The proportion of strains lacking espC and carrying efa1/lifA was higher for patient-derived strains (30.1%) than for strains from healthy individuals (13.2%), but the difference was not significant. Strains carrying both espC and efa1/lifA were rare (2 strains from patients). Statistical analyses revealed significant relationships between espC and yjaA and between efa1/lifA and nleB, as well as significant inverse relationships between espC and efa1/lifA and between efa1/lifA and yjaA. espC was found in eae HMA types a1, a2, and c2, whereas efa1/lifA was found in types b1, b2, and c1. In addition, 6 polymorphisms of espC were found. The espC, yjaA, efa1/lifA, and nleB genes were mutually dependent, and their distributions were related to eae type, findings that should be considered in future epidemiological studies.
机译：研究了除肠上皮细胞受损（LEE）所在地以外的其他致病岛（PAI）上的基因与腹泻的关系。肠致病性大肠埃希氏菌（EPEC），espC保留在EspC PAI上，OI-122基因（ efa1 / lifA < / em>， nleB ），系统发育标记基因 yjaA 和EPEC粘附因子（EAF）上的成束菌毛基因 bfpA 对质粒进行了研究。 E。从腹泻患者（ n = 83）和健康人（）中分离出的带有intimin基因（ eae ）且不含Shiga毒素基因的大肠埃希菌菌株。 n = 38），在日本进行了PCR评估。通过异源双链迁移率分析（HMA）鉴定了 eae 和 espC 的基因型。从带有和不带有腹泻的个体中分离出的携带这些基因的菌株的比例分别为： bfpA ，分别为13.3％和7.9％; espC ，分别为25.3和36.8％; efa1 / lifA ，分别为32.5和13.2％; nleB ，分别为63.9和60.5％; yjaA ，42.2和55.3％。仅对 efa1 / lifA 具有统计学意义（ P <0.05）。来自患者的菌株（30.1％）中缺乏 espC 且携带 efa1 / lifA 的菌株的比例高于健康个体的菌株（13.2％），但是差异不重要。携带 espC 和 efa1 / lifA 的菌株很少（来自患者的2株）。统计分析表明， espC 和 yjaA 之间以及 efa1 / lifA 和 nleB 之间存在显着关系，并且存在显着的逆关系。 espC 和 efa1 / lifA 之间的关系以及 efa1 / lifA 和 yjaA之间的关系。 espC 在 eae HMA类型a1，a2和c2中被发现，而 efa1 / lifA 在b1，b2和c1类型中被发现。另外，发现了 espC 的6个多态性。 espC ， yjaA ， efa1 / lifA 和 nleB 基因是相互依赖的，并且它们的分布与 eae 类型，在未来的流行病学研究中应考虑的发现。

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