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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Analysis of Cases of Italian Sheep Scrapie and Comparison with Cases of Bovine Spongiform Encephalopathy (BSE) and Experimental BSE in Sheep
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Molecular Analysis of Cases of Italian Sheep Scrapie and Comparison with Cases of Bovine Spongiform Encephalopathy (BSE) and Experimental BSE in Sheep

机译：意大利绵羊刮Sc病例的分子分析以及与牛海绵状脑病（BSE）和实验性BSE病例的比较

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Concerns have been raised about the possibility that the bovine spongiform encephalopathy (BSE) agent could have been transmitted to sheep populations via contaminated feedstuffs. The objective of our study was to investigate the suitability of molecular strain typing methods as a surveillance tool for studying scrapie strain variations and for differentiating PrPSc from sheep scrapie, BSE, and sheep BSE. We studied 38 Italian sheep scrapie cases from 13 outbreaks, along with a British scrapie case, an experimental ovine BSE, and 3 BSE cases, by analyzing the glycoform patterns and the apparent molecular masses of the nonglycosylated forms of semipurified, proteinase-treated PrPSc. Both criteria were able to clearly differentiate sheep scrapie from BSE and ovine experimental BSE. PrPSc from BSE and sheep BSE showed a higher glycoform ratio and a lower molecular mass of the nonglycosylated form compared to scrapie PrPSc. Scrapie cases displayed homogeneous PrPSc features regardless of breed, flock, and geographic origin. The glycoform patterns observed varied with the antibody used, but either a monoclonal antibody (MAb) (F99/97.6.1) or a polyclonal antibody (P7-7) was able to distinguish scrapie from BSE PrPSc. While more extensive surveys are needed to further corroborate these findings, our results suggest that large-scale molecular screening of sheep populations for BSE surveillance may be eventually possible.
机译：人们担心牛海绵状脑病（BSE）病原体可能已通过受污染的饲料传播给绵羊种群。我们的研究目的是研究分子菌株分型方法作为监测羊痒病菌株变异以及区分PrP Sc 与绵羊羊痒病，疯牛病和羊疯牛病的监测工具的适用性。我们通过分析半纯化，经蛋白酶处理的PrP < sup> Sc 。这两个标准都能够清楚地区分绵羊瘙痒病与疯牛病和绵羊实验性疯牛病。与痒痒病的PrP Sc 相比，来自疯牛病和绵羊BSE的PrP Sc 显示出更高的糖型比和更低的非糖基化形式的分子量。 rap病例显示出均一的PrP Sc 特征，而与品种，羊群和地理来源无关。观察到的糖型模式随所使用的抗体而异，但是单克隆抗体（MAb）（F99 / 97.6.1）或多克隆抗体（P7-7）能够区分瘙痒病与BSE PrP Sc 。尽管需要更广泛的调查以进一步证实这些发现，但我们的结果表明，最终可能对绵羊种群进行大规模分子筛查以进行BSE监测。

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