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首页> 外文期刊>Journal of Clinical Microbiology >Characterizations of Adenovirus Type 41 Isolates from Children with Acute Gastroenteritis in Japan, Vietnam, and Korea
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Characterizations of Adenovirus Type 41 Isolates from Children with Acute Gastroenteritis in Japan, Vietnam, and Korea

机译：日本，越南和韩国患有急性胃肠炎儿童的41型腺病毒分离株的特征

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摘要

Genetic and antigenic characterizations of 70 strains of adenovirus type 41 (Ad41), isolated between 1998 and 2001 from children in Japan, Vietnam, and Korea, were done by DNA restriction enzyme (RE) analysis, sequencing analysis, and monoclonal antibody (MAb)-based enzyme-linked immunosorbent assay (ELISA). Eight genome types were observed in the present study, among which D25, D26, D27, and D28 were novel genome types. These eight genome types were divided into two genome-type clusters (GTCs) based on phylogenetic analysis of the hypervariable regions (HVRs) of the hexon. GTC1 includes D1, D25, D26, D27, and D28, and the GTC2 contains D4, D12, and D22. The amino acid homologies among the members within a GTC were 97 to 100%, whereas between the members of different GTCs the homologies were 92 to 94%. The specificity of the GTC classification was confirmed by ELISA with MAb 1F, which was selected by the Ad41 prototype Tak strain. It was found that only the isolates of GTC1 but not of GTC2 reacted with MAb 1F. These results suggest that Ad41 isolates from the three countries should be classified into two subtypes. The accumulation of amino acid mutations located in HVRs of hexon are indicative for the classification of Ad41 subtype.
机译：通过DNA限制酶（RE）分析，测序分析和单克隆抗体（MAb）对1998年至2001年之间从日本，越南和韩国的儿童中分离出的70株41型腺病毒（Ad41）进行了遗传和抗原表征。酶联免疫吸附测定（ELISA）。在本研究中观察到八种基因组类型，其中D25，D26，D27和D28是新颖的基因组类型。基于六边形的高变区（HVR）的系统发育分析，将这八种基因组类型分为两个基因组类型簇（GTC）。 GTC1包含D1，D25，D26，D27和D28，而GTC2包含D4，D12和D22。 GTC成员之间的氨基酸同源性为97％至100％，而不同GTC成员之间的氨基酸同源性为92％至94％。 GTC分类的特异性通过MAb 1F的ELISA确认，该抗体由Ad41原型Tak菌株选择。发现只有GTC1的分离物而不是GTC2的分离物与MAb 1F反应。这些结果表明，来自三个国家的Ad41分离株应分为两个亚型。位于六邻体HVR中的氨基酸突变的积累指示Ad41亚型的分类。

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