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首页> 外文期刊>Journal of Clinical Microbiology >“Bacteroides goldsteinii sp. nov.” Isolated from Clinical Specimens of Human Intestinal Origin
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“Bacteroides goldsteinii sp. nov.” Isolated from Clinical Specimens of Human Intestinal Origin

机译：“ Bacteroides goldsteinii sp.。十一月。”从人类肠道来源的临床标本中分离

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Phenotypic and phylogenetic studies were performed on an unknown gram-negative, strictly anaerobic, rod-shaped bacterium isolated from human clinical specimens. This organism was indole negative, resistant to 20% bile, produced acetic and a lesser amount of succinic acids as the major end products of glucose metabolism, and possessed a G+C content of approximately 43 mol%. Comparative 16S rRNA gene sequencing demonstrated that the unidentified bacterium was a member of the Cytophaga-Flavobacter-Bacteroides phylum of gram-negative bacteria and formed a close association (with an average sequence similarity of 93.6%) with the second subcluster of the Porphyromonas cluster in the Bacteroides subgroup. Phylogenetically and phenotypically it resembled Bacteroides merdae; however, a 16S rRNA gene sequence divergence of approximately 5.5% between the unknown bacterium and B. merdae, as well as distinguishable biochemical characteristics, demonstrate that the unknown bacterium is genotypically and phenotypically distinct and represents a previously unknown subline within the Porphyromonas phylogenetic cluster. Furthermore, a DNA-DNA reassociation value of 17.8% between isolates WAL 12034T (the type strain of this novel taxon) and ATCC 43184T (B. merdae type strain) also documented the separateness of the unknown species and B. merdae. Based on the phenotypic and phylogenetic findings, a new species, “Bacteroides goldsteinii sp. nov,” is proposed. The G+C content of the DNA is 43 mol% for Bacteroides. The type strain of “B. goldsteinii” is WAL 12034T (= CCUG 48944T = ATCC BAA-1180T).
机译：对从人类临床标本中分离出的未知的革兰氏阴性，严格厌氧的杆状细菌进行了表型和系统发育研究。该生物是吲哚阴性的，对20％的胆汁有抗性，产生乙酸和少量的琥珀酸作为葡萄糖代谢的主要终产物，并且具有大约43mol％的G + C含量。比较的16S rRNA基因测序表明，未鉴定的细菌是革兰氏阴性细菌的门菌的一个成员，与该细菌形成了紧密的联系（平均序列相似性为93.6％）。拟杆菌属（Bacteroides）子组中的卟啉单胞菌簇的第二个子簇。在系统发育和表型上，它类似于 Bacteroides merdae 。但是，未知细菌与 B之间的16S rRNA基因序列差异约为5.5％。 merdae 以及可区别的生化特征表明，该未知细菌在基因型和表型上是不同的，并且代表了 Porphyromonas 系统发育簇中以前未知的亚系。此外，分离株WAL 12034 T （该新型分类群的类型菌株）与ATCC 43184 T （ B。merdae）之间的DNA-DNA重结合值为17.8％。 型菌株）还记录了未知物种和 B的分离性。 merdae 。根据表型和系统发育的发现，一个新物种“ Bacteroides goldsteinii sp。”。 ”。对于拟杆菌而言，DNA的G + C含量为43 mol％。 “ B”的类型株。 goldsteinii ”是WAL 12034 T （= CCUG 48944 T = ATCC BAA-1180 T ）。

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