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首页> 外文期刊>Journal of Clinical Microbiology >Search for Localized Dysbiosis in Crohn's Disease Ulcerations by Temporal Temperature Gradient Gel Electrophoresis of 16S rRNA
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Search for Localized Dysbiosis in Crohn's Disease Ulcerations by Temporal Temperature Gradient Gel Electrophoresis of 16S rRNA

机译：通过16S rRNA的时间温度梯度凝胶电泳在克罗恩病溃疡中寻找局部性病。

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The mucosa-associated microbiota lining the gut epithelium might play a central role in the activation and/or perpetuation of mucosal inflammation in Crohn's disease (CD). We sought for localized dysbiosis by comparing the biodiversity and composition of the microbiotas in ulcerated and nonulcerated mucosal samples from patients with CD. Biopsy samples (n = 75) of ulcerated and adjacent nonulcerated mucosa were collected during colonoscopy from 15 patients, from the ileum, right colon, left colon, and rectum. Temporal temperature gradient gel electrophoresis (TTGE) of bacterial 16S rRNAs was used to evaluate the dominant bacterial species. TTGE profiles were compared using software that calculates similarity percentages. For a given patient, average similarity indexes between ulcerated and nonulcerated mucosal TTGE profiles ranged from 95.2% ± 4.2% to 97.9% ± 1.7% (means ± standard deviations) for the different segments. The mean values did not differ significantly. Average interindividual similarity indexes for a given segment among the different patients ranged from 33.6% ± 15.5% to 42.0% ± 25.6%. In CD, the dominant microbiotas do not differ qualitatively between ulcerated and nonulcerated mucosae. Biodiversity remains high in ulcerated mucosa. This argues against a pathogenic role of localized qualitative dysbiosis in CD-associated ulceration.
机译：肠道上皮内衬的与黏膜相关的微生物群可能在克罗恩病（CD）的黏膜炎症的激活和/或持久中起着核心作用。我们通过比较CD患者溃疡性和非溃疡性黏膜样品的生物多样性和微生物群组成，寻求局部性营养不良。在结肠镜检查期间，从回肠，右结肠，左结肠和直肠的15例患者中收集溃疡和邻近的未溃疡的黏膜的活检样本（ n = 75）。细菌16S rRNA的时间温度梯度凝胶电泳（TTGE）用于评估优势细菌种类。使用计算相似度百分比的软件比较了TTGE配置文件。对于给定的患者，不同段的溃疡性和非溃疡性粘膜TTGE图谱之间的平均相似性指数范围从95.2％±4.2％到97.9％±1.7％（均值±标准差）。平均值无显着差异。在不同患者中，给定区段的平均个体间相似性指数为33.6％±15.5％至42.0％±25.6％。在CD中，溃疡性和非溃疡性粘膜之间的优势菌群在质量上没有差异。溃疡性粘膜的生物多样性仍然很高。这与CD相关性溃疡中的定性定性营养不良的致病作用相反。

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