Development and Use of an Internal Positive Control for Detection of Bordetella pertussis by PCR

Stéphanie Herwegh; Christophe Carnoy; Frédéric Wallet; Caroline Lo?ez; René J. Courcol

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Development and Use of an Internal Positive Control for Detection of Bordetella pertussis by PCR

机译：PCR检测百日咳博德特氏菌内部阳性对照的开发和应用

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An internal control of amplification was constructed by recombinant PCR to detect PCR inhibitors. This exogenous DNA was included in the reaction mixture and coamplified with the target gene. This detection was successfully applied to the diagnosis of whooping cough by amplification of a fragment of Bordetella pertussis IS481.

机译：通过重组PCR构建扩增的内部对照，以检测PCR抑制剂。该外源DNA包括在反应混合物中，并与靶基因共扩增。通过扩增百日咳博德特氏菌 IS 481 片段，这种检测成功地用于百日咳的诊断。 展开▼

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《Journal of Clinical Microbiology》 |2005年第5期|共3页

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Stéphanie Herwegh; Christophe Carnoy; Frédéric Wallet; Caroline Lo?ez; René J. Courcol;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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1. Addition of a Homologous Internal Control to a Real-Time PCR Assay for Detection of Bordetella pertussis [J] . Christoph Koidl, Egon Marth, Gerhard Mühlbauer, Clinical Chemistry: Journal of the American Association for Clinical Chemists . 2005,第12期

机译：在实时PCR检测中添加同源内部对照以检测百日咳博德特氏菌

2. Evaluation of RealStar Bordetella pertussis PCR Kit 1.0 for Qualitative Detection and Differentiation of Bordetella pertussis and Bordetella parapertussis Specific DNA in Respiratory Samples [J] . Rottengatter K., Wolff D., Gerritzen A. The Journal of molecular diagnostics: JMD . 2017,第6期

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3. Development of a multiplex real-time PCR assay for the detection of Bordetella pertussis and Bordetella parapertussis in a single tube reaction. [J] . Arbefeville S., Levi M. H., Ferrieri P. Journal of Microbiological Methods . 2014,第Null期

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4. Development of an internal control plasmid used for mycobacterial detection by PCR [C] . Brey BJ, Radcliff RP, Clark Jr DL, International Colloquium on Paratuberculosis . 2007

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6. Development and Use of an Internal Positive Control for Detection of Bordetella pertussis by PCR [O] . Stéphanie Herwegh, Christophe Carnoy, Frédéric Wallet, 2005

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7. Development and Use of an Internal Positive Control for Detection of Bordetella pertussis by PCR [O] . Herwegh, Stéphanie, Carnoy, Christophe, Wallet, Frédéric, 2005

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8. Multiplex PCR for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in Clinical Specimens [R] . McDonough, E. A. , Barrozo, C. P. , Russell, K. L. , 2005

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Development and Use of an Internal Positive Control for Detection of Bordetella pertussis by PCR

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