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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of Hepatitis C Virus NS5b and 5′ Noncoding Gene Sequencing Methods in a Multicenter Study
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Comparison of Hepatitis C Virus NS5b and 5′ Noncoding Gene Sequencing Methods in a Multicenter Study

机译：一项多中心研究比较丙型肝炎病毒NS5b和5'非编码基因测序方法

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A national evaluation study was performed in 11 specialized laboratories with the objective of assessing their capacities to genotype hepatitis C virus (HCV) and define the applicability of a given genotyping method. The panel consisted of 14 samples positive for HCV RNA of different genotypes (including 3 samples with two different artificially mixed genotypes) and 1 HCV-negative sample. Seventeen sets of data were gathered from the 11 participating laboratories. The sensitivities ranged from 64.3 to 100% and from 42.7 to 85.7% for the methods that used sequencing of the NS5b region and the 5′ noncoding (5′ NC) region, respectively. When the data for the artificially mixed samples were excluded, NS5b genotyping gave correct results for 80% of the samples, 1.7% of the samples were misclassified, and 18.3% of the samples had false-negative results. By 5′ NC-region genotyping methods, 58.3% of the results were correct, 29.7% were incomplete, 8.3% were misclassifications, 1.2% were false positive, and 2.4% were false negative. Only two procedures based on NS5b sequencing correctly identified one of the three samples with mixtures of genotypes; the other methods identified the genotype corresponding to the strain with the highest viral load in the sample. Our results suggest that HCV 5′ NC-region genotyping methods give sufficient information for clinical purposes, in which the determination of the subtype is not essential, and that NS5b genotyping methods are more reliable for subtype determination, which is required in epidemiological studies.
机译：在11个专门实验室中进行了一项国家评估研究，目的是评估其对丙型肝炎病毒（HCV）进行基因分型的能力并确定给定基因分型方法的适用性。该小组由14个对不同基因型的HCV RNA呈阳性的样品（包括3个具有两种不同人工混合基因型的样品）和1个HCV阴性样品组成。从11个参与实验室收集了17套数据。对于使用NS5b区和5'非编码（5'NC）区测序的方法，灵敏度分别为64.3％至100％和42.7％至85.7％。当排除人工混合样本的数据时，NS5b基因分型为80％的样本提供了正确的结果，1.7％的样本被错误分类，而18.3％的样本具有假阴性结果。通过5'NC区基因分型方法，正确的结果为58.3％，不完全的结果为29.7％，分类错误的为8.3％，假阳性的为1.2％，假阴性的为2.4％。只有基于NS5b测序的两种方法可以正确地鉴定出三种具有基因型混合物的样品中的一种。其他方法鉴定了与样品中病毒载量最高的菌株相对应的基因型。我们的研究结果表明，HCV 5'NC区基因分型方法为临床目的提供了足够的信息，其中亚型的确定不是必不可少的，而NS5b基因分型方法对于亚型的确定更可靠，这在流行病学研究中是必需的。

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