...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Identification and Characterization of Variable-Number Tandem-Repeat Markers for Typing of Brucella spp.
【24h】

Identification and Characterization of Variable-Number Tandem-Repeat Markers for Typing of Brucella spp.

机译：布鲁氏菌属分型的可变数目串联重复标记的鉴定和表征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Members of the genus Brucella infect many domesticated and wild animals and cause serious zoonotic infection in humans. The availability of discriminatory molecular typing tools to inform and assist conventional epidemiological approaches would be invaluable in controlling these infections, but efforts have been hampered by the genetic homogeneity of the genus. We report here on a molecular subtyping system based on 21 variable-number tandem-repeat (VNTR) loci consisting of 13 previously unreported loci and 8 loci previously reported elsewhere. This approach was applied to a collection of 121 Brucella isolates obtained worldwide and representing all six classically recognized Brucella species. The size of repeats selected for inclusion varied from 5 to 40 bp giving VNTR loci with a range of diversities. The number of alleles detected ranged from 2 to 21, and Simpson's diversity index values ranged from 0.31 to 0.92. This assay divides the 121 isolates into 119 genotypes, and clustering analysis results in groups that, with minor exceptions, correspond to conventional species designations. Reflecting this, the use of six loci in isolation was shown to be sufficient to determine species designation. On the basis of the more variable loci, the assay could also discriminate isolates originating from restricted geographical sources, indicating its potential as an epidemiological tool. Stability studies carried out in vivo and in vitro showed that VNTR profiles were sufficiently stable such that recovered strains could readily be identified as the input strain. The method described here shows great potential for further development and application to both epidemiological tracing of Brucella transmissions and in determining relationships between isolates worldwide.
机译：Brucella 属的成员感染许多驯养和野生动物，并引起人类严重的人畜共患病感染。在控制这些感染方面，提供有区别的分子分型工具来告知和协助常规流行病学方法将是无价的，但是该类的遗传同质性阻碍了这种努力。我们在这里报告基于21个可变数目的串联重复（VNTR）基因座的分子亚型系统，该基因座由13个先前未报告的基因座和8个先前在其他地方报道的基因座组成。此方法应用于在全球范围内获得的121个 Brucella 分离株的集合，这些分离株代表了所有六个经典认可的 Brucella 物种。选择用于包含的重复序列的大小在5至40 bp之间变化，从而使VNTR基因座具有一定范围的多样性。检测到的等位基因数量在2到21之间，辛普森的多样性指数值在0.31到0.92之间。该测定法将121个分离株分为119个基因型，并且聚类分析结果分为几类，除少数例外，与常规物种名称相对应。反映这一点的事实是，单独使用六个基因座已足以确定物种名称。在更具可变性的基因座的基础上，该测定法还可区分源自受限地理来源的分离株，表明其作为流行病学工具的潜力。在体内和体外进行的稳定性研究表明，VNTR谱图足够稳定，因此可以容易地将回收的菌株鉴定为输入菌株。本文描述的方法在进一步发展和应用布鲁氏菌传播的流行病学追踪以及确定全球分离株之间的关系方面显示出巨大的潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号