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首页> 外文期刊>Journal of Clinical Microbiology >Quantitative Detection of Plasma Human Immunodeficiency Virus Type 2 Subtype A RNA by the Nuclisens EasyQ Assay (Version 1.1)
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Quantitative Detection of Plasma Human Immunodeficiency Virus Type 2 Subtype A RNA by the Nuclisens EasyQ Assay (Version 1.1)

机译：通过Nuclisens EasyQ分析法（1.1版）定量检测血浆人类免疫缺陷病毒2型亚型RNA

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No commercial viral load assay has yet been approved for use for measurement of human immunodeficiency virus type 2 (HIV-2) RNA levels in plasma. We assessed the performance of the NucliSens EasyQ (version 1.1) assay (EasyQ; bioMérieux, Boxtel, The Netherlands) to quantify HIV-2 viremia. A viral stock was prepared from an HIV-2 (subtype A)-infected patient. Culture supernatant was subjected to viral particle counting by electron microscopy. Serial dilutions of the viral stock were made in HIV-negative plasma and were used to test EasyQ for its sensitivity, linearity, and reproducibility. RNA was quantified by the NucliSens EasyQ (version 1.1) assay. Plasma samples from 75 HIV-2-infected patients were further tested. EasyQ was able to quantify HIV-2 RNA in a reproducible manner. Overall, estimates of the number of HIV-2 RNA copies/ml obtained with EasyQ were lower than those obtained by electron microscopy; however, the differences were always less than 0.7 log (mean, 0.55 ± 0.19 log10). The assay showed good linearity (r2 = 0.964; P < 0.0001). The agreement between both measures was assessed by use of a Bland-Altman plot; the narrow limits (0.158 to 0.952), defined as the mean difference ± 2 standard deviations, indicated good agreement. The reproducibility was also good, since the between-run coefficients of variation were 1.49, 3.60, and 12.25% for samples containing 6.30, 4.30, and 2.30 log10 HIV-2 RNA copies/ml, respectively. HIV-2 RNA was detected in 34 of 75 (45%) plasma specimens (mean, 2.72 log RNA copies/ml; range, 1.74 to 4.11 log RNA copies/ml); the rest of the specimens were considered to have undetectable viremia. A negative correlation was found between the number of HIV-2 RNA copies/ml and CD4 counts. In summary, EasyQ was shown to be reliable for the measurement of plasma HIV-2 subtype A RNA levels and may be a feasible tool for routine clinical monitoring of HIV-2 subtype A-infected patients.
机译：尚无商业病毒载量测定法被批准用于测量血浆中2型人类免疫缺陷病毒（HIV-2）RNA水平。我们评估了NucliSens EasyQ（1.1版）分析（EasyQ;bioMérieux，Boxtel，荷兰）的性能，以量化HIV-2病毒血症。从感染了HIV-2（A型）的患者制备病毒原液。通过电子显微镜对培养物上清液进行病毒颗粒计数。在HIV阴性血浆中制备病毒原液的系列稀释液，并用于测试EasyQ的灵敏度，线性和可重复性。通过NucliSens EasyQ（1.1版）测定法定量RNA。进一步测试了来自75名感染HIV-2的患者的血浆样本。 EasyQ能够以可重复的方式定量HIV-2 RNA。总体而言，通过EasyQ获得的HIV-2 RNA拷贝数/毫升估计值低于通过电子显微镜获得的估计值;但是，差异始终小于0.7 log（平均值为0.55±0.19 log 10 ）。该测定法显示出良好的线性（ r 2 = 0.964; P <0.0001）。两种方法之间的一致性是通过使用布兰德-奥特曼图进行评估的;窄限（0.158至0.952）定义为均差±2标准偏差，表明一致性良好。重复性也很好，因为对于每次分别含有6.30、4.30和2.30 log 10 HIV-2 RNA拷贝/ ml的样品，两次运行之间的变异系数分别为1.49、3.60和12.25％。 75个血浆样本中有34个（45％）检测到HIV-2 RNA（平均值为2.72 log RNA拷贝/毫升;范围为1.74至4.11 log RNA拷贝/毫升）;其余标本被认为具有不可检测的病毒血症。发现HIV-2 RNA拷贝数/ ml与CD4计数之间呈负相关。总之，EasyQ被证明对血浆HIV-2亚型A RNA水平的测量是可靠的，并且可能是对HIV-2亚型A感染患者进行常规临床监测的可行工具。

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