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首页> 外文期刊>Journal of Clinical Microbiology >Use of Spoligotyping and Large Sequence Polymorphisms To Study the Population Structure of the Mycobacterium tuberculosis Complex in a Cohort Study of Consecutive Smear-Positive Tuberculosis Cases in The Gambia
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Use of Spoligotyping and Large Sequence Polymorphisms To Study the Population Structure of the Mycobacterium tuberculosis Complex in a Cohort Study of Consecutive Smear-Positive Tuberculosis Cases in The Gambia

机译：在冈比亚连续性涂片阳性结核病例队列研究中，利用Spoligotyping和大序列多态性研究结核分枝杆菌复合体的种群结构

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Mycobacterium africanum, first described in Senegal in 1968, causes up to half of the smear-positive pulmonary tuberculosis cases in West Africa, but it has not been found in other geographical areas except among recent West African migrants. The reasons for the geographic restriction of M. africanum are unknown. We used molecular tools to determine the population structure of the Mycobacterium tuberculosis complex in a cohort study of consecutive smear-positive tuberculosis cases in The Gambia. We collected and genotyped 386 clinical isolates using spoligotype analysis and PCRs for large sequence polymorphisms (LSPs) and compared the genotype patterns to the patterns in an international database. The results of spoligotyping and LSP analysis for the study population were also compared to determine the correlation between them. The main lineages within the Mycobacterium tuberculosis complex identified in The Gambia included M. africanum type I (38.4%), characterized by an LSP in region of difference 702 (RD702; West African type 2). Among the M. tuberculosis sensu stricto isolates, lineages characterized by RD182 and by RD174 were the most common. We also detected a gradient in the prevalence of M. africanum that extended from neighboring Guinea-Bissau. The genotypic diversity of the spoligotype patterns was greater among the isolates of M. africanum than among the isolates of M. tuberculosis. We postulate that M. africanum became endemic in West Africa first, before the introduction of different lineages within M. tuberculosis sensu stricto.
机译：非洲分枝杆菌最早于1968年在塞内加尔描述，在西非造成多达一半的涂片阳性肺结核病例，但除最近的西非移民外，在其他地理区域均未发现。 M的地理限制的原因。 africanum 是未知的。在冈比亚连续涂片阳性结核病例的队列研究中，我们使用分子工具确定了结核分枝杆菌复合体的种群结构。我们使用spoligotype分析和PCR对大序列多态性（LSPs）进行了收集，并对386个临床分离株进行了基因分型，并将其基因型模式与国际数据库中的模式进行了比较。还比较了研究人群的基因分型和LSP分析的结果，以确定它们之间的相关性。在冈比亚确定的结核分枝杆菌复合体内的主要谱系包括 M。 I型非洲菊（38.4％），其特征在于差异702（RD702;西非2型）地区的LSP。在 M中。严格意义上的结核分枝杆菌，以RD182和RD174为特征的谱系是最常见的。我们还检测到 M患病率存在​​梯度。从邻国几内亚比绍开始的非洲种。在 M分离株中，spoligotype模式的基因型多样性更大。非洲菊比 M的分离株结核病。我们假设该 M。 africanum 首先在西非流行，然后在 M中引入了不同的血统。结核病。

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