...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Development of a Multilocus Microsatellite Typing Approach for Discriminating Strains of Leishmania (Viannia) Species
【24h】

Development of a Multilocus Microsatellite Typing Approach for Discriminating Strains of Leishmania (Viannia) Species

机译：鉴别利什曼原虫（维尼亚）物种菌株的多位点微卫星分型方法的开发。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A multilocus microsatellite typing (MLMT) approach based on the analysis of 15 independent loci has been developed for the discrimination of strains belonging to different Viannia species. Thirteen microsatellite loci were isolated de novo from microsatellite-enriched libraries for both Leishmania braziliensis and L. guyanensis. Two previously identified markers, AC01 and AC16, were modified and added to our marker set. Markers were designed to contain simple dinucleotide repeats flanked by the minimal possible number of nucleotides in order to allow variations in repeat numbers to be scored as size variations of the PCR products. The 15 markers in total were amplified for almost all of the strains of Viannia tested; one marker did not amplify from the two L. peruviana strains included in the study. When 30 strains of L. braziliensis, 21 strains of L. guyanensis, and 2 strains of L. peruviana were tested for polymorphisms, all strains except two strains of L. guyanensis had individual MLMT types. Distance-based analysis identified three main clusters. All strains except one strain of L. guyanensis grouped together. Two clusters consisted of strains of L. braziliensis according to their geographical origins. The two strains of L. peruviana grouped together with strains of L. braziliensis from Peru and the adjacent Brazilian state of Acre. MLMT has proven capable of individualizing strains even from the same areas of endemicity and of detecting genetic structures at different levels. MLMT is thus applicable for epidemiological and population genetic studies of strains within the subgenus Viannia.
机译：基于15个独立基因座的分析，已开发出一种多基因座微卫星分型（MLMT）方法，用于区分属于不同种类的 Viannia 的菌株。从巴西细小利什曼原虫（Leishmania braziliensis）和 L的富含微卫星的文库中重新分离了13个微卫星基因座。古安族。修改了两个先前确定的标记AC01和AC16，并将其添加到我们的标记集中。标记被设计为包含简单的二核苷酸重复序列，其侧翼是核苷酸的最小可能数目，以便允许将重复数目的变化记为PCR产物的大小变化。几乎所有测试过的 Viannia 菌株均扩增了15个标记;一个标记未从两个 L中扩增。研究中包括了peruviana 菌株。当30株 L。 braziliensis ，是21个 L菌株。 Guyanensis 和2个 L菌株。测试了Peruviana 的多态性，除两个 L菌株外，所有菌株。 Guyanensis 具有单独的MLMT类型。基于距离的分析确定了三个主要集群。除一株 L外的所有菌株。 Guyanensis 分组在一起。两个簇由 L菌株组成。根据巴西巴西的地理起源。两种 L菌株。 peruviana 与 L菌株组合在一起。来自秘鲁和邻近的巴西阿克州的braziliensis 。事实证明，MLMT甚至能够从相同的地方性流行区中分离出菌株，并能够检测不同水平的遗传结构。因此，MLMT可用于 Viannia 属下菌株的流行病学和群体遗传研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号