...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Comparison of a New Neuraminidase Detection Assay with an Enzyme Immunoassay, Immunofluorescence, and Culture for Rapid Detection of Influenza A and B Viruses in Nasal Wash Specimens
【24h】

Comparison of a New Neuraminidase Detection Assay with an Enzyme Immunoassay, Immunofluorescence, and Culture for Rapid Detection of Influenza A and B Viruses in Nasal Wash Specimens

机译：一种新的神经氨酸酶检测方法与酶免疫分析，免疫荧光法和用于快速检测鼻洗样本中甲型和乙型流感病毒的培养物的比较

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The performance of a new, rapid, easy-to-perform assay based on neuraminidase enzyme activity for detection of influenza virus types A and B was compared to detection by culture, indirect immunofluorescence, and enzyme immunoassay in 479 nasal wash specimens from children with respiratory infections. Compared to isolation of influenza virus by culture, the neuraminidase assay had a sensitivity of 70.1%, specificity of 92.4%, positive predictive value of 76.3%, and negative predictive value of 89.9%. There was a higher sensitivity for the detection of influenza A virus (76.4%) than for influenza B virus (40.9%). Indirect immunofluorescence showed a sensitivity of 59.8% and specificity of 97% compared to culture isolation for detection of influenza A and B viruses. Enzyme immunoassay showed a sensitivity of 89.7% and specificity of 98.1% for the detection of influenza A alone. The quality of the nasal wash specimen had a significant effect on the detection of influenza virus by all of the assays. A strong response of the neuraminidase assay was more likely to represent a culture-confirmed influenza infection. This new rapid neuraminidase assay was useful for the detection of influenza A and B viruses in nasal wash specimens.
机译：将一种基于神经氨酸酶活性的新型快速，简便测定方法用于检测A和B型流感病毒的性能与通过培养，间接免疫荧光和酶联免疫吸附法检测的479例呼吸道儿童鼻洗液的检测结果进行了比较感染。与通过培养分离流感病毒相比，神经氨酸酶测定法的敏感性为70.1％，特异性为92.4％，阳性预测值为76.3％，阴性预测值为89.9％。甲型流感病毒的检测灵敏度（76.4％）比乙型流感病毒的检测灵敏度（40.9％）更高。与用于检测甲型和乙型流感病毒的培养物分离相比，间接免疫荧光法的灵敏度为59.8％，特异性为97％。酶免疫法检测甲型流感病毒的灵敏度为89.7％，特异性为98.1％。鼻洗标本的质量对所有检测方法对流感病毒的检测都有重要影响。神经氨酸酶测定的强烈反应更可能代表培养物确认的流感感染。这种新的快速神经氨酸酶测定法可用于检测洗鼻标本中的甲型和乙型流感病毒。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号