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首页> 外文期刊>Journal of Clinical Microbiology >Characterization of Lyme Borreliosis Isolates from Patients with Erythema Migrans and Neuroborreliosis in Southern Sweden
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Characterization of Lyme Borreliosis Isolates from Patients with Erythema Migrans and Neuroborreliosis in Southern Sweden

机译：瑞典南部红斑偏头痛和神经性贝氏体病患者莱姆病的分离

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Southern Sweden is an area of Lyme borreliosis (LB) endemicity, with an incidence of 69 cases per 100,000 inhabitants. The most frequent clinical manifestations are erythema migrans (77%) and neuroborreliosis (16%). There was no record of humanBorrelia strains being isolated from patients in this region before the prospective study reported here. Borreliaspirochetes were isolated from skin and cerebrospinal fluid (CSF) from LB patients living in the region. A total of 39 strains were characterized by OspA serotype analysis, species-specific PCR, and signature nucleotide analysis of the 16S rRNA gene. Of 33 skin isolates, 31 (93.9%) were Borrelia afzelii strains and 2 (6.1%) were Borrelia garinii strains. Of six CSF isolates, five (83.3%) were B. garinii and one (16.7%) wasB. afzelii. Neither Borrelia burgdorferisensu stricto strains nor multiple infections were observed. TheB. afzelii isolates were of OspA serotype 2. ThreeB. garinii strains were of OspA serotype 5, and the remaining four strains were of OspA serotype 6. All of theB. garinii strains belonged to the same 16S ribosomal DNA ribotype class. Our findings agree with earlier findings from other geographic regions in Europe where B. afzelii and B. garinii have been recovered predominately from skin and CSF cultures, respectively. To further study the possible presence in Sweden of the genotype B. burgdorferi sensu stricto, which is known to be present in Europe and to occur predominately in patients with Lyme arthritis, molecular detection of Borrelia-specific DNA in synovial samples from Lyme arthritis patients should be performed.
机译：瑞典南部是莱姆病（Lyme borreliosis，LB）流行地区，每100,000居民中有69例发病。最常见的临床表现是红斑偏头痛（77％）和神经性贝氏体病（16％）。在此前瞻性研究报道之前，尚无从该地区患者中分离出人类 Borrelia 菌株的记录。从居住在该地区的LB患者的皮肤和脑脊液（CSF）中分离出螺旋体。通过OspA血清型分析，物种特异性PCR和16S rRNA基因的特征核苷酸分析对总共39个菌株进行了表征。在33株皮肤分离物中，有31株（93.9％）是短小孢子菌（Borrelia afzelii），而2株（6.1％）是短小孢子菌。在六个CSF分离株中，有五个（83.3％）是 B。 garinii ，其中1个（16.7％）是 B。 afzelii 。既没有发现 sensu stricto菌株，也没有发现多发感染。 B。 afzelii 分离株为OspA血清型2。三个 B。 garinii菌株为OspA血清型5，其余四个菌株为OspA血清型6。所有 B。 garinii 菌株属于同一类16S核糖体DNA核糖型。我们的发现与欧洲其他地理区域（ B）的早期发现一致。 afzelii 和 B。 garinii 主要从皮肤和CSF培养物中回收。为了进一步研究在瑞典可能存在基因型B的情况。 burgdorferi sensu stricto，已知在欧洲存在，主要发生在莱姆关节炎患者中，应该对莱姆关节炎患者滑膜样品中的特异DNA进行分子检测。

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