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首页> 外文期刊>Journal of Clinical Microbiology >Comparison and Evaluation of Osiris and Sirscan 2000 Antimicrobial Susceptibility Systems in the Clinical Microbiology Laboratory
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Comparison and Evaluation of Osiris and Sirscan 2000 Antimicrobial Susceptibility Systems in the Clinical Microbiology Laboratory

机译：临床微生物学实验室中Osiris和Sirscan 2000抗菌药敏系统的比较和评估

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The Osiris and Sirscan 2000 systems are two semiautomated systems that can be used to read and interpret the results on disk diffusion agar plates. They are both used for determination of susceptibility to antimicrobial agents. The present study compared both systems versus the NCCLS standard method of visual reading with a ruler. Both inpatient and outpatient samples with a total of 315 nonfastidious gram-negative strains were obtained. In total, 3,724 organism-antimicrobial agent combinations that fulfilled the NCCLS guidelines for disk diffusion susceptibility testing were evaluated prospectively. The results obtained with both systems in comparison with those obtained by the classical nonautomated means of interpretation were excellent, with correlation coefficients of 0.96 for both systems. The overall agreements for susceptibility interpretation were 96.56 and 96.24% with the Osiris and Sirscan systems, respectively. Very major errors were obtained for 8 (1.07%) and 10 (1.34%) organism-antimicrobial agent combinations with the Osiris and Sirscan systems, respectively. In addition, major errors were obtained for 2 (0.07%) and 6 (0.21%) combinations with the Osiris and Sirscan systems, respectively. Minor errors were obtained for 118 and 124 organism-antimicrobial agent combinations with the Osiris and Sirscan systems, respectively. Overall, both the Osiris system and the Sirscan system are comparable and reliable systems for determination of interpretative categories from the zone diameters of standard disk diffusion test plates.
机译：Osiris和Sirscan 2000系统是两个半自动化系统，可用于读取和解释磁盘扩散琼脂板上的结果。它们都用于确定对抗菌剂的敏感性。本研究比较了这两种系统与NCCLS标准用直尺进行视觉阅读的方法。总共获得了315株非耐病革兰氏阴性菌株的住院和门诊样品。总共评估了3,724种符合NCCLS磁盘扩散敏感性测试指南的生物体-抗菌剂组合。与通过经典的非自动化解释方法获得的结果相比，使用这两种系统获得的结果都非常好，两种系统的相关系数均为0.96。 Osiris和Sirscan系统的敏感性解释的总体协议分别为96.56％和96.24％。对于Osiris和Sirscan系统，分别有8种（1.07％）和10种（1.34％）的有机物-抗菌剂组合获得了非常大的误差。另外，使用Osiris和Sirscan系统分别获得2个（0.07％）和6个（0.21％）组合的主要误差。 Osiris和Sirscan系统分别对118种和124种有机体-抗菌剂组合获得了较小的误差。总体而言，Osiris系统和Sirscan系统都是可比较且可靠的系统，用于从标准圆盘扩散测试板的区域直径确定解释类别。

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