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首页> 外文期刊>Journal of Clinical Microbiology >Random Amplified Polymorphic DNA and Phenotyping Analysis of Salmonella enterica Serovar Enteritidis Isolates Collected from Humans and Poultry in Uruguay from 1995 to 2002
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Random Amplified Polymorphic DNA and Phenotyping Analysis of Salmonella enterica Serovar Enteritidis Isolates Collected from Humans and Poultry in Uruguay from 1995 to 2002

机译：1995年至2002年在乌拉圭从人类和家禽中收集的肠道沙门氏菌肠炎沙门氏菌分离株的随机扩增多态性DNA和表型分析

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Molecular and phenotyping techniques were applied to study Salmonella enterica serovar Enteritidis strains both from human cases of infection and of avian origin isolated in Uruguay from 1995 to 2002. A group of 62 isolates was subjected to random amplified polymorphic DNA (RAPD) assay and analysis of antibiotic resistance patterns. Twenty-one of these strains were further characterized by phage typing and analysis of their protein expression profiles. RAPD fingerprinting with five different primers discriminated 10 different genetic profiles. Of the 62 strains tested, 48 had a single major genetic profile, whereas the other nine profiles were evenly distributed among the other strains. The genetic diversity was greater among strains of animal origin than among isolates of human origin. Comparative examination of the results obtained by RAPD analysis and phenotypic analysis and by strain source provided evidence of the reliable discriminatory power of RAPD analysis in our study. Six avian isolates with antibiotic resistance were detected: two were nalidixic acid resistant and four had a particular β-lactam resistance pattern. The last four isolates all had the same unusual phage type (phage type 4b); however, RAPD analysis differentiated them into two groups. Two isolates with unique RAPD profiles were recovered from distinct human cases, suggesting that the technique differentiates unrelated strains. Overall, the results show the existence of a predominant genetic type that is present in poultry and that is transmitted to humans. There are also several other genotypes, but only a few of them could be recovered from human sources, suggesting the existence of different pathogenic traits among strains circulating in the country.
机译：从1995年至2002年在乌拉圭分离的人感染和禽源感染病例中，应用分子和表型技术研究肠炎沙门氏菌肠炎沙门氏菌菌株。对62个分离株进行随机扩增的多态性DNA分析（RAPD）分析和抗生素耐药性模式分析。这些菌株中的二十一个通过噬菌体分型和对其蛋白表达谱的分析来进一步表征。用五种不同引物进行的RAPD指纹图谱可区分10种不同的遗传图谱。在所测试的62个菌株中，有48个具有单一的主要遗传图谱，而其他9个图谱在其他菌株之间平均分布。在动物起源品系中，遗传多样性要比在人类起源品系中更大。通过RAPD分析和表型分析以及菌株来源对结果进行的比较检验，为我们研究中RAPD分析的可靠鉴别力提供了证据。检测到六株具有抗生素抗性的禽分离株：两株耐萘啶酸，四株具有特定的β-内酰胺抗性。最后四个分离株均具有相同的异常噬菌体类型（噬菌体类型4b）;但是，RAPD分析将它们分为两组。从不同的人类病例中回收了两个具有独特RAPD谱的分离株，表明该技术区分了无关菌株。总体而言，结果表明存在于禽类中并传播给人类的主要遗传类型。还有其他几种基因型，但只能从人源中回收，这表明该国流通的菌株之间存在不同的致病性状。

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